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作为成虫的死胡同陷阱:一种保护柑橘园的潜在生物学策略。

as a dead-end trap for adult : A potential biological tactic for protecting citrus orchards.

作者信息

Zheng Lixia, Xu Qianqian, Gong Gu, Liao Yonglin, Yu Min, Shabala Sergey, Chen Wensheng, Wu Weijian

机构信息

International Research Center for Environmental Membrane Biology and Department of Horticulture, Foshan University, Foshan, China.

Laboratory of Insect Ecology, South China Agricultural University, Guangzhou, China.

出版信息

Front Plant Sci. 2023 Jan 6;13:1081663. doi: 10.3389/fpls.2022.1081663. eCollection 2022.

Abstract

The Asian citrus psyllid, Kuwayama (Hemiptera: Liviidae), is a key vector of the causal agents of Huanglongbing (HLB), a devastating disease affecting citrus almost worldwide. L. is an important commercial crop in China. Field observations suggested that adults die on leaves when grown nearby citrus orchards. In this study, the preference for and survivorship of adults on and their feeding behavior were investigated. The results showed that adults were attracted to and to the green leaf volatiles (GLVs) ()-3-hexenol and ()-2-hexenol. The survival of adults on was less than 30 h, which was shorter than that for adults without food (35 h) and on a suitable host L. (29 days). Electrical penetration graph (EPG) recordings revealed that the pathway phase of on leaves consisted of four waveforms-the non-probing phase (NP), the pathway phase (PP, including intercellular probing of activity in the phloem (C) and phloem penetration (D)), phloem salivation (E1), and phloem ingestion (E2). only secreted saliva and ingested sap from phloem on leaves and spent the longest duration in phloem sap ingestion (E2). Moreover, L-nicotine, an important defense compound against insects in plants, was highly toxic to . These results suggested that plants could help to sustainably control the spread of and HLB when growing in and around citrus orchards.

摘要

亚洲柑橘木虱,Kuwayama(半翅目:木虱科),是黄龙病(HLB)病原体的关键传播媒介,黄龙病是一种几乎影响全球柑橘的毁灭性疾病。柑橘在中国是一种重要的经济作物。田间观察表明,成虫在柑橘园附近生长时会在叶片上死亡。在本研究中,调查了成虫对柑橘和九里香的偏好、在其上的存活情况及其取食行为。结果表明,成虫被柑橘和绿叶挥发物(GLVs)(E)-3-己烯醇和(Z)-2-己烯醇所吸引。成虫在九里香上的存活时间不到30小时,这比没有食物的成虫(35小时)和在合适寄主酸橙上的成虫(29天)要短。刺吸电位图谱(EPG)记录显示,成虫在九里香叶上的路径阶段由四种波形组成——非刺探阶段(NP)、路径阶段(PP,包括细胞间刺探、韧皮部活动(C)和韧皮部穿透(D))、韧皮部唾液分泌(E1)和韧皮部取食(E2)。成虫仅在九里香叶上从韧皮部分泌唾液并摄取汁液,且在韧皮部汁液摄取(E2)阶段花费的时间最长。此外,L-尼古丁是柑橘植物中一种重要的抗虫防御化合物,对亚洲柑橘木虱具有高毒性。这些结果表明,九里香植物在柑橘园及其周边种植时有助于可持续地控制亚洲柑橘木虱和黄龙病的传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e028/9853912/08749b159f34/fpls-13-1081663-g001.jpg

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