Rigter Pomme M F, de Konink Charlotte, van Woerden Geeske M
Department of Clinical Genetics, Erasmus Medical Center, Rotterdam, Netherlands.
Erfelijke Neuro-Cognitieve Ontwikkelingsstoornissen, Expertise Centre for Neurodevelopmental Disorders, Erasmus Medical Center, Rotterdam, Netherlands.
Front Neurosci. 2023 Jan 6;16:1086994. doi: 10.3389/fnins.2022.1086994. eCollection 2022.
The gamma subunit of calcium/calmodulin-dependent protein kinase 2 (CAMK2G) is expressed throughout the brain and is associated with neurodevelopmental disorders. Research on the role of CAMK2G is limited and attributes different functions to specific cell types.
To further expand on the role of CAMK2G in brain functioning, we performed extensive phenotypic characterization of a knockout mouse.
We found different CAMK2G isoforms that show a distinct spatial expression pattern in the brain. Additionally, based on our behavioral characterization, we conclude that CAMK2G plays a minor role in hippocampus-dependent learning and synaptic plasticity. Rather, we show that CAMK2G is required for motor function and that the loss of CAMK2G results in impaired nest-building and marble burying behavior, which are innate behaviors that are associated with impaired neurodevelopment.
Taken together, our results provide evidence for a unique function of this specific CAMK2 isozyme in the brain and further support the role of CAMK2G in neurodevelopment.
钙/钙调蛋白依赖性蛋白激酶2（CAMK2G）的γ亚基在整个大脑中表达，并与神经发育障碍有关。关于CAMK2G作用的研究有限，且将不同功能归因于特定细胞类型。
为了进一步拓展CAMK2G在脑功能中的作用，我们对一只基因敲除小鼠进行了广泛的表型特征分析。
我们发现了不同的CAMK2G亚型，它们在大脑中呈现出独特的空间表达模式。此外，基于我们的行为特征分析，我们得出结论，CAMK2G在海马体依赖的学习和突触可塑性中起次要作用。相反，我们表明CAMK2G对运动功能是必需的，并且CAMK2G的缺失会导致筑巢和埋大理石行为受损，这些是与神经发育受损相关的先天行为。
综上所述，我们的结果为这种特定的CAMK2同工酶在大脑中的独特功能提供了证据，并进一步支持了CAMK2G在神经发育中的作用。
