Chen Huan, Kang Zhewei, Liu Xueqing, Zhao Yinglin, Fang Zeman, Zhang Jinling, Zhang Handi
Department of Psychiatry, Shantou University Mental Health Center, Shantou, China.
Institute of Mental Health, Peking University Sixth Hospital, Beijing, China.
Front Neurosci. 2023 Jan 4;16:1074631. doi: 10.3389/fnins.2022.1074631. eCollection 2022.
Social stress in adolescents precipitates stress-related emotional disorders. In this study we aimed to investigate oligodendrogenesis in three stress-associated brain regions, medial prefrontal cortex (mPFC), habenula, and amygdala in adolescent mice exposed to social defeat stress.
Four-week-old adolescent mice were subjected to social defeat for 10 days, followed by behavioral tests and evaluations of oligodendroglial proliferation and differentiation.
Stressed mice showed reduced social interaction, more stretched approach posture, lower sucrose preference, but no changes in the forced swimming test. EdU labeled proliferative cells, newly formed NG2EdU + oligodendrocyte precursor cells (OPCs), and Olig2EdU oligodendrocyte lineage cells (OLLs) were significantly decreased in the mPFC and the lateral habenula, but not in the amygdala and the medial habenula in socially defeated mice. APCEdu newly-generated mature oligodendrocytes (OLs) were decreased in the mPFC in stressed mice. However, the total number of NG2 OPCs, APC mature OLs, and Olig2 OLLs were comparable in all the brain regions examined between stressed and control mice except for a decrease of APC mature OLs in the prelimbic cortex of stressed mice.
Our findings indicate that adolescent social stress causes emotion-related behavioral changes and region-specific impairment of oligodendrogenesis.
青少年的社会压力会引发与压力相关的情绪障碍。在本研究中，我们旨在调查遭受社会挫败压力的青春期小鼠的三个与压力相关的脑区，即内侧前额叶皮质（mPFC）、缰核和杏仁核中的少突胶质细胞生成情况。
对四周龄的青春期小鼠进行为期10天的社会挫败，随后进行行为测试以及少突胶质细胞增殖和分化的评估。
应激小鼠表现出社交互动减少、接近姿势更伸展、蔗糖偏好降低，但在强迫游泳测试中没有变化。在遭受社会挫败的小鼠中，EdU标记的增殖细胞、新形成的NG2EdU +少突胶质前体细胞（OPC）和Olig2EdU少突胶质细胞谱系细胞（OLL）在mPFC和外侧缰核中显著减少，但在杏仁核和内侧缰核中没有减少。应激小鼠mPFC中的APCEdu新生成的成熟少突胶质细胞（OL）减少。然而，除了应激小鼠前边缘皮质中APC成熟OL减少外，在应激小鼠和对照小鼠检查的所有脑区中，NG2 OPC、APC成熟OL和Olig2 OLL的总数相当。
我们的研究结果表明，青少年社会压力会导致与情绪相关的行为变化以及少突胶质细胞生成的区域特异性损伤。
