Gonzalez M F, Sharp F R
Department of Neurology, University of California, San Francisco.
J Neurosci. 1987 Oct;7(10):2991-3001. doi: 10.1523/JNEUROSCI.07-10-02991.1987.
Fetal frontal cortex from 18-d-old embryonic rat brain was transplanted into cavities of juvenile host motor/sensory cortex. Two to seven months later, sections were reacted for NADPH-diaphorase (NADPH-d) enzyme histochemistry. NADPH-d-positive neurons survived in 11 of 13 grafts. All but one of the transplants had reduced numbers of these neurons, although in 3 transplants the reductions were moderate and not statistically significant. The distribution and morphology of NADPH-d neurons within most grafts was comparable to that of NADPH-d neurons in normal host cortex. At the margin of the 2 transplants with no NADPH-d neuronal perikarya, NADPH-d fibers crossed from host to transplant as far as a millimeter into the transplant, and on rare occasions, the host neurons that gave rise to these fibers were identified. This suggests that host-transplant interactions are possible. One transplant had an abnormally large number of NADPH-d-positive neurons and fibers, possibly due to selective survival of these neurons. The data reported here for NADPH-d in cortical transplants may also apply to neuropeptide Y (NPY), since nearly all neocortical NPY neurons also contain NADPH-d.
将18日龄胚胎大鼠脑的胎儿额叶皮质移植到幼年宿主运动/感觉皮质的腔隙中。两到七个月后，切片进行烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）酶组织化学反应。13个移植物中有11个的NADPH-d阳性神经元存活。除了一个移植物外，所有移植物中这些神经元的数量都减少了，尽管在3个移植物中减少程度适中且无统计学意义。大多数移植物中NADPH-d神经元的分布和形态与正常宿主皮质中的NADPH-d神经元相似。在2个没有NADPH-d神经元胞体的移植物边缘，NADPH-d纤维从宿主穿过到移植物中，深入移植物达1毫米，在极少数情况下，还能识别出产生这些纤维的宿主神经元。这表明宿主与移植物之间可能存在相互作用。一个移植物中NADPH-d阳性神经元和纤维数量异常多，可能是由于这些神经元的选择性存活。这里报道的皮质移植物中NADPH-d的数据可能也适用于神经肽Y（NPY），因为几乎所有新皮质NPY神经元也都含有NADPH-d。
