Sharp F R, Gonzalez M F, Sagar S M
Department of Neurology, University of California, San Francisco.
J Neurosci. 1987 Oct;7(10):3002-15. doi: 10.1523/JNEUROSCI.07-10-03002.1987.
Fetal frontal cortex transplants that survived 2-9 months in cavities in adult rat motor/sensory cortex were processed for vasoactive intestinal polypeptide (VIP), somatostatin 14 (SS), and neuropeptide Y (NPY) immunocytochemistry, and NADPH-diaphorase (NADPH-d) histochemistry. All transplants had surviving VIP, SS, NPY, and NADPH-d neuronal perikarya and fibers with normal adult morphology. The number of peptidergic neurons within transplants, however, often appeared to be less than that in equivalent areas of host cortex. Most transplanted SS and VIP neuronal perikarya did not migrate to form the laminae characteristic of normal cortex. A few transplants had SS and VIP cells arranged in laminae in which the VIP processes were parallel to one another and perpendicular to one transplant surface, approximating normal host neocortex. VIP, NPY, and SS fibers crossed between host brains and transplants, suggesting that peptide host-transplant interactions are possible. All adult host cortical and most transplanted NPY neurons colocalized with NADPH-d. The failure of some transplanted NPY neurons to express NADPH-d suggests these transplanted cells may be functionally impaired, but that they can survive without the NADPH-d enzyme.
在成年大鼠运动/感觉皮层腔隙中存活2至9个月的胎儿额叶皮层移植物，进行了血管活性肠肽（VIP）、生长抑素14（SS）、神经肽Y（NPY）免疫细胞化学以及还原型辅酶Ⅱ黄递酶（NADPH-d）组织化学处理。所有移植物均有存活的VIP、SS、NPY以及NADPH-d神经元胞体和纤维，其形态为正常成年形态。然而，移植物中肽能神经元的数量往往似乎少于宿主皮层同等区域的数量。大多数移植的SS和VIP神经元胞体并未迁移形成正常皮层特有的层状结构。少数移植物中有呈层状排列的SS和VIP细胞，其中VIP突起相互平行且垂直于一个移植物表面，近似于正常宿主新皮层。VIP、NPY和SS纤维在宿主脑与移植物之间交叉，表明肽类物质在宿主与移植物之间可能存在相互作用。所有成年宿主皮层和大多数移植的NPY神经元与NADPH-d共定位。一些移植的NPY神经元未能表达NADPH-d，提示这些移植细胞可能功能受损，但它们在没有NADPH-d酶的情况下仍能存活。
