下丘脑背内侧的γ-氨基丁酸能神经元调节七氟醚麻醉下的意识状态。

GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia.

作者信息

Wang Yanfeng, Song Yanping, Tong Li, Wang Lu, Cao Jiangbei, Qin Gang, Liu Xingyang, Mi Weidong, Wang E, Guo Yongxin

机构信息

Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.

Department of Anesthesia, 922 Hospital of PLA, Hengyang, Hunan 421002, China.

出版信息

iScience. 2022 Dec 30;26(1):105913. doi: 10.1016/j.isci.2022.105913. eCollection 2023 Jan 20.

The neural inhibitory gamma-aminobutyric acid (GABA) system in the regulation of anesthetic consciousness is heterogeneous, and the medial hypothalamus (MH), consisting of ventromedial hypothalamus (VMH) and dorsomedial hypothalamus (DMH), plays an important role in sleep and circadian rhythm. However, the role of MH GABAergic neurons (MH) in anesthesia remains unclear. In this study, we used righting reflex, electroencephalogram (EEG), and arousal behavioral score to evaluate the sevoflurane anesthesia. Activation of MH or DMH neurons prolonged the anesthesia induction time, shortened the anesthesia emergence time, and induced EEG arousal and body movement during anesthesia; meanwhile, VMH neurons activated only induced EEG changes during 1.5% sevoflurane anesthesia. Furthermore, inhibition of DMH neurons significantly deepened sevoflurane anesthesia. Therefore, DMH neurons exert a strong emergence-promoting effect on induction, maintenance, and arousal during sevoflurane general anesthesia, which helps to reveal the mechanism of anesthesia.

神经抑制性γ-氨基丁酸（GABA）系统在麻醉意识调节中具有异质性，由腹内侧下丘脑（VMH）和背内侧下丘脑（DMH）组成的内侧下丘脑（MH）在睡眠和昼夜节律中起重要作用。然而，MH的GABA能神经元（MH）在麻醉中的作用仍不清楚。在本研究中，我们使用翻正反射、脑电图（EEG）和觉醒行为评分来评估七氟醚麻醉。激活MH或DMH神经元会延长麻醉诱导时间，缩短麻醉苏醒时间，并在麻醉期间诱发EEG觉醒和身体运动；同时，仅激活VMH神经元只会在1.5%七氟醚麻醉期间引起EEG变化。此外，抑制DMH神经元会显著加深七氟醚麻醉。因此，DMH神经元在七氟醚全身麻醉的诱导、维持和觉醒过程中发挥强大的促苏醒作用，这有助于揭示麻醉机制。