第三脑室室管膜下细胞的个体发生。
Ontogeny of ependymoglial cells lining the third ventricle in mice.
机构信息
Department of Biomedical Sciences, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
出版信息
Front Endocrinol (Lausanne). 2023 Jan 5;13:1073759. doi: 10.3389/fendo.2022.1073759. eCollection 2022.
INTRODUCTION
During hypothalamic development, the germinative neuroepithelium gives birth to diverse neural cells that regulate numerous physiological functions in adulthood.
METHODS
Here, we studied the ontogeny of ependymal cells in the mouse mediobasal hypothalamus using the BrdU approach and publicly available single-cell RNAseq datasets.
RESULTS
We observed that while typical ependymal cells are mainly produced at E13, tanycyte birth depends on time and subtypes and lasts up to P8. Typical ependymocytes and β tanycytes are the first to arise at the top and bottom of the dorsoventral axis around E13, whereas α tanycytes emerge later in development, generating an outside-in dorsoventral gradient along the third ventricle. Additionally, α tanycyte generation displayed a rostral-to-caudal pattern. Finally, tanycytes mature progressively until they reach transcriptional maturity between P4 and P14.
DISCUSSION
Altogether, this data shows that ependyma generation differs in time and distribution, highlighting the heterogeneity of the third ventricle.
简介
在下丘脑发育过程中，原始神经上皮产生多种调节成年后多种生理功能的神经细胞。
方法
在这里，我们使用 BrdU 方法和公开的单细胞 RNAseq 数据集研究了小鼠中脑基底下丘脑室管膜细胞的个体发生。
结果
我们观察到，虽然典型的室管膜细胞主要在 E13 时产生，但 tanycyte 的产生取决于时间和亚型，并持续到 P8。典型的室管膜细胞和β tanycytes 首先在 E13 左右沿背腹轴的顶部和底部出现，而α tanycytes 在发育后期出现，沿着第三脑室产生从外向内的背腹梯度。此外，α tanycyte 的产生呈现出从头到尾的模式。最后，tanycytes 逐渐成熟，直到在 P4 和 P14 之间达到转录成熟。
讨论
总之，这些数据表明室管膜的产生在时间和分布上存在差异，突出了第三脑室的异质性。
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