亚种BL-99与低聚果糖通过调节肠道微生物群产生的协同排便作用。
Synergistic defecation effects of subsp. BL-99 and fructooligosaccharide by modulating gut microbiota.
作者信息
Zhang Qi, Zhao Wen, Luo Jie, Shi Shaoqi, Niu Xiaokang, He Jian, Wang Yicheng, Zeng Zhaozhong, Jiang Qiuyue, Fang Bing, Chen Juan, Li Yixuan, Wang Fuqing, He Jingjing, Guo Jie, Zhang Ming, Zhang Liwei, Ge Shaoyang, Hung Wei-Lian, Wang Ran
机构信息
Key Laboratory of Functional Dairy, Co-Constructed by Ministry of Education and Beijing Municipality, Department of Nutrition and Health, China Agricultural University, Beijing, China.
College of Food Science and Technology, Hunan Agricultural University, Changsha, China.
出版信息
Front Immunol. 2025 Jan 9;15:1520296. doi: 10.3389/fimmu.2024.1520296. eCollection 2024.
INTRODUCTION
Synbiotics have revealed the possibility of improving constipation through gut microbiota. The synergistic efficacy of subsp. lactis BL-99 (BL-99) and fructooligosaccharide (FOS) on constipation have not been investigated.
METHODS
Loperamide-induced constipated mice model was established to explore the effect of BL-99, FOS, and BL-99+FOS on changes of defecation-related parameters, gut microbiota and metabolites.
RESULTS AND DISCUSSION
The results showed that BL-99, FOS, and BL-99+FOS each alleviated constipation, with the synbiotic showing significant efficacy in the first black stool defecation time, fecal number, fecal weight, and the gastrointestinal transit rate ( < 0.05). Additionally, significant increased in serum 5-HT and IL-10 were observed in the BL-99+FOS group, alongside an increased relative abundance of , and , while significantly reducing the relative abundance of and . These changes facilitated alterations in short-chain fatty acids (SCFAs) metabolism, and were closely associated with the expression of genes related to the 5-HT pathway and the modulation of serum inflammatory factors. This study provides a theoretical basis for BL-99 and FOS synbiotics to improve constipation by regulating the gut microbiota and metabolites.
引言
合生元已显示出通过肠道微生物群改善便秘的可能性。乳酸亚种BL-99（BL-99）和低聚果糖（FOS）对便秘的协同功效尚未得到研究。
方法
建立洛哌丁胺诱导的便秘小鼠模型，以探究BL-99、FOS和BL-99+FOS对排便相关参数、肠道微生物群和代谢产物变化的影响。
结果与讨论
结果表明，BL-99、FOS和BL-99+FOS均能缓解便秘，合生元在首次排黑便时间、粪便数量、粪便重量和胃肠传输率方面显示出显著功效（<0.05）。此外，在BL-99+FOS组中观察到血清5-羟色胺和白细胞介素-10显著增加，同时[具体菌属名称1]、[具体菌属名称2]和[具体菌属名称3]的相对丰度增加，而[具体菌属名称4]和[具体菌属名称5]的相对丰度显著降低。这些变化促进了短链脂肪酸（SCFAs）代谢的改变，并与5-羟色胺途径相关基因的表达和血清炎症因子的调节密切相关。本研究为BL-99和FOS合生元通过调节肠道微生物群和代谢产物改善便秘提供了理论依据。
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