National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Nutrients. 2023 Aug 26;15(17):3741. doi: 10.3390/nu15173741.
Slow transit constipation (STC) is a prevalent gastrointestinal condition with slow transit, and some probiotics can effectively relieve constipation, but the exact mechanisms have not been fully understood. In this study, we evaluate the impact of GUANKE (GUANKE) on diphenoxylate-induced slow transit constipation and speculate on the underlying mechanisms in a mouse model. Administration of GUANKE alleviated constipation indexes, including defecation time, fecal output and water content, and gastrointestinal transit ratio. In addition, GUANKE restored the protein expression of constipation-related intestinal factors (aquaporins (AQPs) and interstitial Cajal cells (ICCs)) in colon tissues measured using immunofluorescence staining; regulated the neurotransmitters and hormones, such as increased levels of 5-hydroxytryptamine, substance P, and motilin; and decreased levels of vasoactive intestinal peptide and nitric oxide in serum, as measured by an ELISA. 16S rRNA and correlation analysis of feces indicated that GUANKE administration effectively reduced constipation-induced enrichment and suggested a potential contribution of to diphenoxylate-induced STC in mice. GUANKE had no effect on short-chain fatty acids (SCFAs) in cecum content. This study revealed that GUANKE may alleviate constipation in mice through regulating intestinal neurotransmitter and hormone release and altering specific bacterial taxa, rather than by affecting SCFAs and the diversity of microbiota in the gut. Further research is needed to confirm if the findings observed in this study will be consistent in other animal studies or clinical trials.
慢传输型便秘（STC）是一种常见的胃肠道疾病，其特点是传输缓慢。一些益生菌可以有效缓解便秘，但确切的机制尚未完全阐明。在本研究中，我们评估了 GUANKE（贯科）对地芬诺酯诱导的慢传输型便秘的影响，并在小鼠模型中推测其潜在机制。GUANKE 的给药缓解了便秘指标，包括排便时间、粪便产量和含水量以及胃肠道传输比。此外，GUANKE 通过免疫荧光染色恢复了便秘相关肠因子（水通道蛋白（AQPs）和间质 Cajal 细胞（ICCs））在结肠组织中的蛋白表达；通过 ELISA 调节了神经递质和激素，如 5-羟色胺、P 物质和胃动素水平的升高；以及血管活性肠肽和一氧化氮水平的降低。16S rRNA 和粪便相关性分析表明，GUANKE 的给药有效减少了便秘诱导的富集，这表明在小鼠中，GUANKE 可能对地芬诺酯诱导的 STC 有潜在贡献。GUANKE 对盲肠内容物中的短链脂肪酸（SCFAs）没有影响。本研究表明，GUANKE 可能通过调节肠道神经递质和激素的释放以及改变特定的细菌分类群来缓解小鼠的便秘，而不是通过影响 SCFAs 和肠道微生物群的多样性。需要进一步的研究来确认在这项研究中观察到的结果是否会在其他动物研究或临床试验中一致。
