Fukui H, Murakami M, Yoshikawa H, Takada K, Muranishi S
Department of Biopharmaceutics, Kyoto Pharmaceutical University, Japan.
J Pharmacobiodyn. 1987 Jun;10(6):236-42. doi: 10.1248/bpb1978.10.236.
The effect of oleic acid-HCO60 mixed micelles (MM) on the absorption of colloidal particles by the large intestine was investigated using the in situ closed loops of rats. Two species of colloidal particles with different sizes, colloidal inks (Platinum ink 2.4-10 nm, Rotring ink 100-800 nm) and colloidal gold particles (mean particle size 5, 20, 40 nm) were chosen. Macroscopic observation showed that in the case of Platinum ink the staining of the mucus of the whole large intestine was promoted by MM and the staining of the rectum remained even after removing the mucus layer. The regional lymph nodes and the thoracic lymph was also stained. On the other hand, in the case of Rotring ink, the stain in the large intestine after removing the mucus layer, in lymph nodes and in the thoracic duct lymph was not observed. The results of quantitative experiments using colloidal gold particles indicated: 1) the upper size limit of colloidal particles absorbed by the large intestine, under the influence of MM was approximately 40 nm, 2) colloidal gold particles were transported from the intestine selectively into the lymphatics and accumulated in the regional lymph nodes, 3) the absorption of colloidal gold particles was largely promoted by MM in the rectum area.
使用大鼠原位闭袢法研究了油酸-HCO60混合胶束（MM）对大肠吸收胶体颗粒的影响。选用了两种不同大小的胶体颗粒，胶体墨水（铂墨水2.4 - 10纳米，红环墨水100 - 800纳米）和胶体金颗粒（平均粒径5、20、40纳米）。宏观观察表明，对于铂墨水，MM促进了整个大肠黏液的染色，即使去除黏液层后直肠仍有染色。区域淋巴结和胸导管淋巴液也有染色。另一方面，对于红环墨水，去除黏液层后在大肠、淋巴结和胸导管淋巴液中均未观察到染色。使用胶体金颗粒的定量实验结果表明：1）在MM影响下，大肠吸收的胶体颗粒的尺寸上限约为40纳米，2）胶体金颗粒从肠道选择性转运至淋巴管并在区域淋巴结中蓄积，3）MM在直肠区域极大地促进了胶体金颗粒的吸收。
