Department of Pathology & Immunology, Baylor College of Medicine; Center for Drug Discovery, Baylor College of Medicine.
Departments of Molecular Virology and Microbiology and Medicine, Baylor College of Medicine.
J Vis Exp. 2023 Jan 6(191). doi: 10.3791/64448.
Endometrial tissue lines the inner cavity of the uterus and is under the cyclical control of estrogen and progesterone. It is a tissue that is composed of luminal and glandular epithelium, a stromal compartment, a vascular network, and a complex immune cell population. Mouse models have been a powerful tool to study the endometrium, revealing critical mechanisms that control implantation, placentation, and cancer. The recent development of 3D endometrial organoid cultures presents a state-of-the-art model to dissect the signaling pathways that underlie endometrial biology. Establishing endometrial organoids from genetically engineered mouse models, analyzing their transcriptomes, and visualizing their morphology at a single-cell resolution are crucial tools for the study of endometrial diseases. This paper outlines methods to establish 3D cultures of endometrial epithelium from mice and describes techniques to quantify gene expression and analyze the histology of the organoids. The goal is to provide a resource that can be used to establish, culture, and study the gene expression and morphological characteristics of endometrial epithelial organoids.
子宫内膜组织位于子宫的内腔，受雌激素和孕激素的周期性控制。它是一种由腔上皮和腺上皮、基质区室、血管网络和复杂的免疫细胞群组成的组织。小鼠模型一直是研究子宫内膜的有力工具，揭示了控制着床、胎盘形成和癌症的关键机制。最近，3D 子宫内膜类器官培养的发展提供了一个先进的模型，可以剖析子宫内膜生物学的信号通路。从基因工程小鼠模型中建立子宫内膜类器官，分析其转录组，并以单细胞分辨率可视化其形态，是研究子宫内膜疾病的重要工具。本文概述了从小鼠中建立 3D 培养的子宫内膜上皮类器官的方法，并描述了定量基因表达和分析类器官组织学的技术。其目的是提供一种资源，可以用来建立、培养和研究子宫内膜上皮类器官的基因表达和形态特征。
