Laboratoire des Microorganismes et Biomolécules Actives Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisie.
Service de Microbiologie et d'Immunologie, Ecole Nationale de Médecine Vétérinaire, Univ. Manouba, 2020 Sidi Thabet, Ariana, Tunisie.
Lett Appl Microbiol. 2023 Jan 23;76(1). doi: 10.1093/lambio/ovac033.
The rates of antibiotic resistance in extraintestinal pathogenic Escherichia coli (ExPEC) have increased significantly in recent years. This study aims at studying antibiotic resistance, virulence factors (VFs), and the phylogenetic background of ExPEC isolated from Palestinian patients. A total of 42 ExPEC isolates were collected from patients with extraintestinal infections in three Palestinian hospitals. Antimicrobial susceptibility was studied by the disk diffusion method. Resistance/virulence-gene profiles, phylogenetic groups, and integron profiles of these isolates were studied by PCR. The susceptibility to carbapenems was detected in 90.5% of the isolates. Half of the isolates were resistant to ampicillin and sulfamethoxazole/trimethoprim, and 33.3% of the isolates were multidrug-resistant. BlaTEM-1 was detected in seven isolates and blaOXA-1 was identified in one isolate. Sul, qnrA, and aac(6')-Ib-cr genes were found in 12, 3, and 2 isolates, respectively. Class 1 integron has been identified in 10 isolates with the gene cassette arrangement dhfr17 + aadA5. The isolates were distributed between phylogroups B2 and D. The presence of VFs, antibiotic resistance genes, and class 1 integron associated with phylogenetic groups (B2 and D) among multidrug-resistant (MDR)-ExPEC recovered from urinary tract infections (UTIs) patients will complicate infection management and increase therapy failure. Routine screening of antibiotic resistance is important for appropriate and efficient empirical treatment.
近年来，肠外致病性大肠杆菌（ExPEC）的抗生素耐药率显著上升。本研究旨在研究从巴勒斯坦患者中分离出的 ExPEC 的抗生素耐药性、毒力因子（VF）和系统发育背景。从三家巴勒斯坦医院的肠外感染患者中收集了总共 42 株 ExPEC 分离株。采用纸片扩散法研究了抗菌药物敏感性。通过 PCR 研究了这些分离株的耐药/毒力基因谱、系统发育群和整合子谱。90.5%的分离株对碳青霉烯类药物敏感。一半的分离株对氨苄西林和磺胺甲恶唑/甲氧苄啶耐药，33.3%的分离株为多药耐药。7 株分离株中检测到 BlaTEM-1，1 株分离株中鉴定出 blaOXA-1。12、3 和 2 株分离株中分别发现了 Sul、qnrA 和 aac(6')-Ib-cr 基因。10 株分离株中鉴定出携带 dhfr17+aadA5 基因盒排列的 I 类整合子。分离株分布于 B2 和 D 群。从尿路感染（UTI）患者中分离出的多药耐药（MDR）-ExPEC 中存在与系统发育群（B2 和 D）相关的 VF、抗生素耐药基因和 I 类整合子，这将使感染管理复杂化并增加治疗失败的风险。常规进行抗生素耐药性筛查对于进行适当和有效的经验性治疗非常重要。
