Department of Microbiology, Biomedical Sciences Institute II, Universidade de São Paulo, 05508-900 São Paulo, Brazil; Laboratory of Molecular and Cellular Biology, Universidade Paulista, 04057-000 São Paulo, Brazil.
Department of Microbiology, Biomedical Sciences Institute II, Universidade de São Paulo, 05508-900 São Paulo, Brazil.
Vet Microbiol. 2014 Jun 25;171(1-2):242-7. doi: 10.1016/j.vetmic.2014.03.027. Epub 2014 Mar 30.
Urinary tract infection (UTI) is a frequent disease of humans and pets and has extra-intestinal pathogenic Escherichia coli (ExPEC) strains as one of the main etiologic agent. ExPEC are characterized by specific virulence factors and are related to a heterogeneous group of human and animal disorders, besides to be a relevant participant in the dissemination of antimicrobial resistance. The purpose of this study was to characterize E. coli strains isolated from UTI of dogs and cats for serotypes, virulence markers, phylogenetic groups and sensitivity to antimicrobial drugs. E. coli was identified as the etiologic agent of UTI in urine samples of 43 pets (7 cats and 36 dogs). Serogroups O2, O4 and O6 corresponded to more than one third of the isolates, being 62% of the total strains classified as B2, 18% as D, 16% as B1 and 4% as A. The iucD (22%), fyuA (80%), traT (51%) and cvaC (20%) genes were distributed among the four phylogenetic groups, whereas the papC/papEF (47%) and malX (67%) genes were found only in groups B2 and D. There were a high number of resistant strains, with 76% of the strains belonging to groups A, B1 and D characterized as multidrug resistant (MDR), whereas only 21% had this phenotype in the group B2. The ExPEC strains isolated in this study displayed pathotypic and phylogenetic similarities with human isolates and high percentages of drug resistance. The finding of MDR ExPEC strains suggests implications for animal and public health and deserves more investigations.
尿路感染（UTI）是人类和宠物的常见疾病，其病原体之一是肠外致病性大肠杆菌（ExPEC）菌株。ExPEC 具有特定的毒力因子，与人类和动物的多种疾病有关，并且是抗生素耐药性传播的重要参与者。本研究旨在对犬猫 UTI 分离的大肠杆菌菌株进行血清型、毒力标志物、系统发育群和药敏性分析。从 43 只宠物（7 只猫和 36 只狗）的尿液样本中鉴定出大肠杆菌为 UTI 的病原体。O2、O4 和 O6 血清群占分离株的三分之一以上，其中 62%的总菌株被归类为 B2，18%为 D，16%为 B1，4%为 A。iucD（22%）、fyuA（80%）、traT（51%）和 cvaC（20%）基因分布于四个系统发育群中，而 papC/papEF（47%）和 malX（67%）基因仅存在于 B2 和 D 群中。耐药菌株数量较多，A、B1 和 D 组的 76%菌株为多重耐药（MDR），而 B2 组只有 21%的菌株具有这种表型。本研究分离的 ExPEC 菌株在表型和系统发育上与人类分离株具有相似性，且耐药率较高。MDR ExPEC 菌株的发现提示对动物和公共卫生具有重要影响，值得进一步研究。
