College of Life Sciences, Nanjing Agricultural University, 6 Tongwei Road Nanjing, Jiangsu 210095, China.
College of Life Sciences, Zaozhuang University, Zaozhuang 277100, China.
Lett Appl Microbiol. 2023 Jan 23;76(1). doi: 10.1093/lambio/ovac040.
2,5-Pyridinedicarboxylic acid (2,5-PDA), a natural N-heterocyclic compound and a substitute for production in plastics, was widely distributed in industrial wastewater. However, the biodegradation of 2,5-PDA has been rarely reported. In this study, strain YJ-5, which could utilize 2,5-PDA as the sole carbon source for growth was isolated from pesticide-contaminated soil. Based on the comparative analysis of the 16S rRNA gene sequence, strain YJ-5 was identified as Agrobacterium sp. 2,5-PDA was completely degraded within 7 d and the optimal growth conditions of temperature, pH, and substrate concentration were 30°C, 7.0, and 0.6 mmol-1, respectively. A new intermediate 6-hydroxy-2,5-PDA was determined by UV/VIS spectroscopy and liquid chromatograph coupled time of flight mass spectrometry. When the electron acceptor (2,6-dichlorophenolindophenol) was employed, the 2,5-PDA could be converted by cell extracts of strain YJ-5 cells into 6-hydroxy-2,5-PDA. These results provided new insights for biodegradation on pyridine dicarboxylate.
2,5- 吡啶二甲酸（2,5-PDA）是一种天然的 N-杂环化合物，也是生产塑料的替代品，广泛分布于工业废水中。然而，2,5-PDA 的生物降解很少被报道。本研究从受农药污染的土壤中分离出一株能够利用 2,5-PDA 作为唯一碳源生长的菌株 YJ-5。通过 16S rRNA 基因序列的比较分析，鉴定菌株 YJ-5 为农杆菌属。2,5-PDA 在 7d 内完全降解，最适生长温度、pH 值和底物浓度分别为 30°C、7.0 和 0.6mmol-1。通过紫外/可见光谱和液相色谱-飞行时间质谱联用确定了一种新的中间产物 6-羟基-2,5-PDA。当使用电子受体（2,6-二氯酚靛酚）时，菌株 YJ-5 细胞提取物可将 2,5-PDA 转化为 6-羟基-2,5-PDA。这些结果为吡啶二甲酸的生物降解提供了新的见解。
