评估铜（Cu）纳米颗粒对稻黄单胞菌（Xanthomonas oryzae pv. oryzae）的生物防治活性、促进生长和水稻（Oryza sativa L.）植物生理学的影响。
Assessment of copper (Cu) nanoparticle for their biocontrol activity against Xanthomonas oryzae pv. oryzae, growth promotion, and physiology of rice (Oryza sativa L.) plants.
机构信息
C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Maliba Campus, Bardoli, Surat 394 350, India.
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Raebareli, Lucknow 226002, India.
出版信息
Lett Appl Microbiol. 2023 Jan 23;76(1). doi: 10.1093/lambio/ovac066.
Among the various biotic factors that disrupt crop yield, Xanthomonas oryzae pv oryzae (Xoo) is the most ruinous microbe of rice and causes bacterial leaf blight (BLB) disease. The present study focused on the utilization of copper nanoparticles (Cu-NPs) to control BLB. The copper nanosuspension (259.7 nm) prepared using Na-CMC, CuSO4·7H2O, and NaOH showed effectively inhibited Xoo (65.0 μg/ml). The performance of Cu-NPs in vivo showed enhanced plant attributes (127.9% root length and 53.9% shoot length) compared to the control and CuSO4 treated seedling. Furthermore, Cu-NPs treated seedlings showed 23.01% disease incidence (DI) compared to CuSO4 (85.71%) treated and control plants (91.83%). In addition to enhancing the growth parameters and reducing DI, seed priming with Cu-NPs improved the total chlorophyll content to 36.0% compared to the control. The assessment of antioxidant enzymes such as superoxide dismutase (1.9 U), polyphenol oxidase, peroxidase, and phenylalanine ammonia-lyase (two- to three-fold) in roots and shoots of rice plants revealed significant enhancement in Cu-NPs treated seedlings (P < 0.05). The present study suggests that Cu-NPs can be used to control Xoo and enhance rice growth.
在破坏作物产量的各种生物因素中，稻黄单胞菌（Xanthomonas oryzae pv oryzae，Xoo）是对水稻危害最大的微生物，可引发细菌性条斑病（BLB）。本研究主要关注利用铜纳米颗粒（Cu-NPs）来控制 BLB。使用 Na-CMC、CuSO4·7H2O 和 NaOH 制备的铜纳米悬浮液（259.7nm）有效抑制了 Xoo（65.0μg/ml）。与对照和 CuSO4 处理的幼苗相比，Cu-NPs 在体内的性能表现出增强的植物属性（根长增加 127.9%，茎长增加 53.9%）。此外，与 CuSO4（85.71%）处理和对照植物（91.83%）相比，Cu-NPs 处理的幼苗的病害发生率（DI）为 23.01%。除了增强生长参数和降低 DI 外，Cu-NPs 浸种还将总叶绿素含量提高到 36.0%，而对照为 36.0%。对超氧化物歧化酶（1.9U）、多酚氧化酶、过氧化物酶和苯丙氨酸解氨酶等抗氧化酶在水稻根和茎中的评估显示，Cu-NPs 处理的幼苗显著增强（P<0.05）。本研究表明，Cu-NPs 可用于控制 Xoo 并促进水稻生长。
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