Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan.
School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan.
Lett Appl Microbiol. 2023 Jan 23;76(1). doi: 10.1093/lambio/ovac024.
This study was conducted with a perception that fructose-rich niches may inhabit novel species of lactic acid bacteria that are gaining importance as probiotics and for the production of exopolysaccharides that have applications in food and pharmaceuticals. Recently, some Lactobacillus species have been reclassified as fructophilic lactic acid bacteria due to their preference for fructose over glucose as a carbon source. These bacteria are likely to be found in fructose rich niches such as flower nectar and insects that feed on it. We explored the butterfly gut and acquired a new isolate, designated as F1, of fructophilic lactic acid bacteria, which produces a glucan-type exopolysaccharide. Whole genome sequencing and in silico analysis revealed that F1 has significantly lower average nucleotide identity and DNA-DNA hybridization values as compared to its closest Apilactobacillus neighbors in phylogenetic analysis. Therefore, we declare the isolate F1 as a novel Apilactobacillus species with the proposed name of Apilactobacillus iqraium F1. Genome mining further revealed that F1 harbors genes for exopolysaccharide synthesis and health-promoting attributes. To this end, F1 is the only Apilactobacillus species harboring three diverse α-glucan-synthesis genes that cluster with different types of dextransucrases in the dendrogram. Moreover, many nutritional marker genes, as well as genes for epithelial cell adhesion and antimicrobial synthesis, were also detected suggesting the probiotic attributes of F1. Overall analysis suggests A. iqraium sp. F1 be a potential candidate for various health beneficial and pharmaceutical applications.
本研究的出发点是，富含果糖的小生境中可能存在新型的乳酸菌，这些乳酸菌作为益生菌的重要性日益增加，并且可以生产具有食品和制药应用前景的胞外多糖。最近，由于某些乳杆菌物种更喜欢果糖而不是葡萄糖作为碳源，因此它们被重新归类为嗜果糖乳酸杆菌。这些细菌可能存在于富含果糖的小生境中，如花蜜和以花蜜为食的昆虫中。我们探索了蝴蝶肠道，并获得了一种新的嗜果糖乳酸菌分离株，命名为 F1，它可以产生一种葡聚糖型胞外多糖。全基因组测序和计算机分析表明，与系统发育分析中最接近的 Apilactobacillus 近邻相比，F1 的平均核苷酸同一性和 DNA-DNA 杂交值明显较低。因此，我们宣布该分离株 F1 为一种新型的 Apilactobacillus 物种，命名为 Apilactobacillus iqraium F1。基因组挖掘进一步表明，F1 具有合成胞外多糖和促进健康的特性。为此，F1 是唯一一种含有三种不同α-葡聚糖合成基因的 Apilactobacillus 物种，这些基因在系统发育树中与不同类型的葡聚糖蔗糖酶聚类。此外，还检测到许多营养标记基因以及上皮细胞黏附和抗菌合成基因，表明 F1 具有益生菌特性。总体分析表明，A. iqraium sp. F1 可能是各种有益健康和药物应用的潜在候选者。
