不同卵泡发育阶段水牛卵巢组织中生长因子的表达

Expression of growth factors in buffalo ovarian tissue across different follicular developmental stages.

作者信息

Soliman Seham Samir, El-Sheikh Marwa, Taha Dalia A, Hamed Karima A, Khalil Wagdy K B

机构信息

Department of Animal Reproduction and Artificial Insemination, Veterinary Research Institute, National Research Centre (NRC), Dokki, Cairo, 12622, Egypt.

Department of Microbial Biotechnology, Biotechnology Research Institute, National Research Centre (NRC), Dokki, Cairo, 12622, Egypt.

出版信息

Arch Gynecol Obstet. 2025 Jun 21. doi: 10.1007/s00404-025-08090-8.

DOI:10.1007/s00404-025-08090-8

PMID:40542852

Abstract

BACKGROUND

In assisted reproduction, poor ovarian response to stimulation negatively affects oocyte yield and is influenced by genetic factors.

OBJECTIVE

This study aimed to quantify the mRNA expression of key growth markers (BMP15, GDF9, OCT4, and FGFR2) in ovarian tissue according to the developmental stages of the follicle.

METHODS

Samples were collected from ovarian tissue. Gene expression levels were analyzed using RT-qPCR. In addition, ELISA was used to measure the concentrations of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), along with reactive oxygen species (ROS) and malondialdehyde (MDA) as oxidative stress markers.

RESULTS

OCT4 expression was similar in preantral and small follicles but significantly upregulated in medium and large follicles. GDF9 expression and SOD activity were highest in small follicles (P < 0.05). BMP15 levels were significantly elevated in small and medium follicles compared to preantral follicles but remained unchanged in large follicles (P < 0.05). FGFR2 expression increased progressively with follicle size (P < 0.05). GPx activity was directly proportional to follicle size, with the lowest levels in preantral follicles. Conversely, ROS, MDA, and CAT concentrations decreased as follicle size increased.

CONCLUSION

These findings provide insights into the molecular regulation of follicular development in buffalo, which could aid in improving reproductive efficiency in assisted reproduction programs.

摘要

背景

在辅助生殖中，卵巢对刺激反应不良会对卵母细胞产量产生负面影响，且受遗传因素影响。

目的

本研究旨在根据卵泡发育阶段定量分析卵巢组织中关键生长标志物（骨形态发生蛋白15、生长分化因子9、八聚体转录因子4和纤维母细胞生长因子受体2）的mRNA表达。

方法

从卵巢组织中采集样本。使用逆转录定量聚合酶链反应分析基因表达水平。此外，采用酶联免疫吸附测定法测量过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的浓度，以及作为氧化应激标志物的活性氧和丙二醛。

结果

八聚体转录因子4在窦前卵泡和小卵泡中的表达相似，但在中卵泡和大卵泡中显著上调。生长分化因子9的表达和超氧化物歧化酶的活性在小卵泡中最高（P＜0.05）。与窦前卵泡相比，骨形态发生蛋白15水平在小卵泡和中卵泡中显著升高，但在大卵泡中保持不变（P＜0.05）。纤维母细胞生长因子受体2的表达随卵泡大小逐渐增加（P＜0.05）。谷胱甘肽过氧化物酶的活性与卵泡大小成正比，在窦前卵泡中水平最低。相反，活性氧、丙二醛和过氧化氢酶的浓度随着卵泡大小的增加而降低。