Soto-Bielicka Patricia, Tejeda Inés, Peropadre Ana, Hazen María José, Fernández Freire Paloma
Department of Biology (Lab A-110), Faculty of Sciences, Universidad Autónoma de Madrid, C/Darwin 2, 28049 Madrid, Spain.
Department of Biology (Lab A-110), Faculty of Sciences, Universidad Autónoma de Madrid, C/Darwin 2, 28049 Madrid, Spain.
Environ Toxicol Pharmacol. 2023 Mar;98:104072. doi: 10.1016/j.etap.2023.104072. Epub 2023 Jan 20.
The potential interactions between the diverse pollutants that can be released into the environment and the resulting outcomes are a challenging issue that needs to be further examined. This in vitro study was aimed to assess potential toxic effects caused by combined exposure to tetrabromobisphenol A, a flame retardant widely used and frequently detected in aquatic matrices, and commercially available polystyrene nanoparticles as reference material to evaluate nanoplastics risks. Our results, using freshwater fish cell lines and a set of relevant cytotoxicity endpoints including cell viability, oxidative stress, and DNA damage, provide additional mechanistic insights that could help to fully characterize the toxicity profiles of tetrabromobisphenol A and polystyrene nanoparticles. Furthermore, we describe subtle changes in cell viability as well as the generation of oxidative DNA damage after coexposure to subcytotoxic concentrations of the tested pollutants.
可能释放到环境中的各种污染物之间的潜在相互作用以及由此产生的结果是一个具有挑战性的问题，需要进一步研究。这项体外研究旨在评估联合暴露于四溴双酚A（一种广泛使用且经常在水生环境中检测到的阻燃剂）和市售聚苯乙烯纳米颗粒（作为评估纳米塑料风险的参考材料）所引起的潜在毒性作用。我们的研究结果，使用淡水鱼细胞系和一系列相关的细胞毒性终点指标，包括细胞活力、氧化应激和DNA损伤，提供了额外的机制性见解，有助于全面描述四溴双酚A和聚苯乙烯纳米颗粒的毒性特征。此外，我们描述了在共同暴露于亚细胞毒性浓度的受试污染物后，细胞活力的细微变化以及氧化性DNA损伤的产生。
