Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal.
Department of Chemistry & CICECO, University of Aveiro, 3810-193 Aveiro, Portugal.
Environ Toxicol Pharmacol. 2019 Jul;69:57-65. doi: 10.1016/j.etap.2019.03.019. Epub 2019 Mar 29.
There is an increasing concern on the consequences of the presence of micro(nano)plastics to marine organisms. The present study aimed to provide information on the effects of polystyrene nanoplastics (PSNPs) to fish cells alone and combined with human pharmaceuticals, other emerging contaminants, using as biological models marine fish cell lines SAF-1 and DLB-1. Cells were exposed for 24 h to 100 nm PSNPs, starting at 0.001 up to 10 mg/L, to assess effects on viability and activity of catalase (antioxidant defense) and glutathione S-transferases (phase II biotransformation and antioxidant defense). The viability of cells was also evaluated after exposure to human pharmaceuticals alone and combined with PSNPs. Overall, PSNPs failed to be cytotoxic but data proved their ability to alter the toxicity of human pharmaceuticals. DLB-1 was the most sensitive cell line to PSNPs. Data support the use of marine fish cell lines in the study of the effects of micro(nano)plastics.
人们越来越关注微（纳）塑料对海洋生物的影响。本研究旨在利用海洋鱼类细胞系 SAF-1 和 DLB-1 作为生物模型，提供有关聚苯乙烯纳米塑料（PSNPs）单独作用以及与人类药物、其他新兴污染物联合作用对鱼类细胞影响的信息。将细胞暴露于 100nm PSNPs 中 24 小时，起始浓度为 0.001 至 10mg/L，以评估对细胞活力和过氧化氢酶（抗氧化防御）和谷胱甘肽 S-转移酶（II 相生物转化和抗氧化防御）活性的影响。还评估了细胞在单独暴露于人类药物以及与 PSNPs 联合暴露后的活力。总体而言，PSNPs 没有表现出细胞毒性，但数据证明了它们改变人类药物毒性的能力。DLB-1 是对 PSNPs 最敏感的细胞系。数据支持使用海洋鱼类细胞系研究微（纳）塑料的影响。
