School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.
School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China; Efficient Intelligent Sewage Treatment Technology Innovation Center of Shandong Province, Linyi 276000, China; Carbon Neutrality and Eco-Environmental Technology Innovation Center of Qingdao, Qingdao 266071, China.
Bioresour Technol. 2023 Mar;372:128658. doi: 10.1016/j.biortech.2023.128658. Epub 2023 Jan 20.
The combined denitrifying phosphorus removal (DPR) and Anammox process is expected to achieve advanced nutrient removal with low carbon consumption. However, exchanging ammonia/nitrate between them is one limitation. This study investigated the feasibility of conducting DPR in a biofilm reactor to solve that problem. After 46-day anaerobic/aerobic operation, high phosphorus removal efficiency (PRE, 83.15 %) was obtained in the activated sludge (AS) and biofilm co-existed system, in which the AS performed better. Phosphate-accumulating organisms might quickly adapt to the anoxic introduced nitrate, but the following aerobic stage ensured a low effluent orthophosphate (<1.03 mg/L). Because of waste sludge discharging and AS transforming to biofilm, the suspended solids dropped below 60 mg/L on Day 100, resulting in PRE decline (17.17 %) and effluent orthophosphate rise (4.23 mg/L). Metagenomes analysis revealed that Pseudomonas and Thiothrix had genes for denitrification and encoding Pit phosphate transporter, and Candidatus_Competibacter was necessary for biofilm formation.
联合反硝化除磷 (DPR) 和厌氧氨氧化工艺有望以低能耗实现高级脱氮除磷。然而，氨氮/硝酸盐之间的交换是一个限制因素。本研究探讨了在生物膜反应器中进行 DPR 的可行性，以解决该问题。经过 46 天的厌氧/好氧运行，在活性污泥 (AS) 和生物膜共存系统中获得了高磷去除效率 (PRE，83.15%)，其中 AS 表现更好。聚磷菌可能会迅速适应缺氧引入的硝酸盐，但随后的好氧阶段确保了低出水正磷酸盐(<1.03mg/L)。由于排泥和 AS 向生物膜的转化，悬浮固体在第 100 天下降到 60mg/L 以下，导致 PRE 下降(17.17%)和出水正磷酸盐上升(4.23mg/L)。宏基因组分析表明，假单胞菌和硫丝菌具有反硝化和编码 Pit 磷酸盐转运蛋白的基因，而生物膜形成所必需的是 Candidatus_Competibacter。
