Troncone R, Farris E, Donatiello A, Auricchio S
Department of Pediatrics, 2nd Faculty of Medicine, University of Naples, Italy.
J Clin Lab Immunol. 1987 Aug;23(4):179-83.
In vitro spontaneous IgG gliadin antibody production was shown in peripheral blood mononuclear cell (PBMC) cultures from 12/14 patients with active coeliac disease; in most cases no increase and sometimes a marked reduction of the in vitro synthesis was observed after pokeweed mitogen (PWM) addition. Lower levels of gliadin antibodies were also detected in PBMC cultures from 7/12 coeliac patients in remission; in all the cases the synthesis was increased by PWM. In vitro production was confirmed by higher levels in 7-day culture supernatants than in 0-day frozen-thawed cell pellets and by the inhibitory effect of cycloheximide. Spontaneous release of antibodies occurred within the first 3 days of culture, while PWM-induced antibody production reached a plateau after 7-9 days. The analysis of the in vitro gliadin antibody production is a promising technique to assess the regulatory mechanisms involved in the humoral immune response to gliadin.
14例活动性乳糜泻患者中有12例的外周血单核细胞（PBMC）培养显示出体外自发产生IgG麦醇溶蛋白抗体；在大多数情况下，添加商陆有丝分裂原（PWM）后，体外合成未见增加，有时反而显著减少。12例缓解期乳糜泻患者中有7例的PBMC培养中也检测到较低水平的麦醇溶蛋白抗体；在所有病例中，PWM均可增加其合成。通过7天培养上清液中的水平高于0天冻融细胞沉淀中的水平以及环己酰亚胺的抑制作用，证实了体外产生。抗体在培养的前3天内自发释放，而PWM诱导的抗体产生在7 - 9天后达到平台期。体外麦醇溶蛋白抗体产生的分析是一种很有前景的技术，可用于评估参与对麦醇溶蛋白体液免疫反应的调节机制。
