Council for Scientific and Industrial Research - Oil Palm Research Institute, P. O. Box 74, Kade, Ghana.
HJA Africa Limited, Accra, Ghana.
Sci Rep. 2023 Jan 23;13(1):1280. doi: 10.1038/s41598-023-27972-y.
Synthetic fungicides are necessary evil in crop production, their usage cannot be neglected or abandoned but must be alternated/supplemented with other control measures such as cultural, host resistance and biocontrol methods to reduce their detrimental effect on the environment and living organisms. A bioproduct (wood vinegar) was evaluated against oil palm seedling pathogens at CSIR-Oil Palm Research Institute, Kusi at different concentrations and compared with an inorganic fungicide at the manufacturer's recommended dosage. Disease pathogens were isolated from collected diseased leaf samples and pure cultures were established on cPDA. PDA was amended with wood vinegar ranging from 0 to 3.35% and 0.1%v/v of carbendazim as a positive control. Daily colony growth was measured in two diagonal lengths and averages of day 6 and day 7 were used to calculate the inhibition percentage for both pathogens. 11 mm/day was the lowest average growth rate recorded for 2.68% v/v of wood vinegar and 14.17 mm/day on control plate of Curvularia species. There was no significant difference between 0.1%v/v carbendazim, 2.68 and 3.35% v/v against Curvularia species whilst significantly, there was difference between 0.1%v/v carbendazim and 2.68 and 3.35%v/v of wood vinegar against Pestalotiopsis species.
在作物生产中，合成杀菌剂是必要的恶，但不能忽视或放弃其使用，而必须与其他控制措施（如文化、宿主抗性和生物防治方法）交替/补充，以减少其对环境和生物的有害影响。在 CSIR-油棕研究所的 Kusi，一种生物制品（木醋液）在不同浓度下被评估用于对抗油棕幼苗病原菌，并与制造商推荐剂量的无机杀菌剂进行了比较。从收集的患病叶片样本中分离出病原菌，并在 cPDA 上建立纯培养物。在 PDA 中添加木醋液，浓度范围为 0 至 3.35%，并以 0.1%v/v 的多菌灵作为阳性对照。在两个对角线上测量每天的菌落生长情况，并使用第 6 天和第 7 天的平均值来计算两种病原菌的抑制率。对于 Curvularia 种，2.68%v/v 的木醋液和对照板的平均生长速率最低为 11mm/天，而 Curvularia 种的平均生长速率为 14.17mm/天。0.1%v/v 的多菌灵与 2.68%和 3.35%v/v 的木醋液对 Curvularia 种之间没有显著差异，而 0.1%v/v 的多菌灵与 2.68%和 3.35%v/v 的木醋液对 Pestalotiopsis 种之间则有显著差异。
