Chen Fengping, Han Ping, Liu Pengfei, Si Naiguo, Liu Junli, Liu Xili
1] Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou. 350002, China [2] Department of Plant Pathology, China Agricultural University, Beijing 100193, China.
1] Department of Plant Pathology, China Agricultural University, Beijing 100193, China [2] Beijing Research Center for Agricultural Standards and Testing, Beijing 100097, China.
Sci Rep. 2014 Sep 25;4:6473. doi: 10.1038/srep06473.
In in vitro tests with 18 plant pathogens, the fungicide 3-[5-(4-chlorophenyl)-2,3-dimethyl-3-isoxazolidinyl] pyridine (SYP-Z048) was highly effective on inhibiting mycelial growth of various ascomycota and basidiomycota, with EC50 values ranging from 0.008 to 1.140 μg/ml. SYP-Z048 had much weaker activity against growth of oomycota with EC50 values > 100 μg/ml. In a second in vitro test with Monilinia fructicola isolates, SYP-Z048 inhibited mycelial growth (EC50 = 0.013 μg/ml), germ tube elongation (EC50 = 0.007 μg/ml), and sporulation but did not affect spore germination. In a detached pear fruit assay inoculated with M. fructicola isolates, SYP-Z048 showed protective and curative activity. Field tests indicated that SYP-Z048 was an efficacious fungicide for control of brown rot disease in two peach orchards. When applied to a single spot on a tomato leaflet in a compound leaf, SYP-Z048 suppressed the growth of Botrytis cinerea isolates on the rest 4 leaflets, indicating that the fungicide has systemic movement in plant tissues. These results indicate that SYP-Z048 has potential for management of brown rot causing by M. fructicola and other diseases caused by ascomycota and basidiomycota.
在针对18种植物病原体的体外试验中，杀菌剂3-[5-(4-氯苯基)-2,3-二甲基-3-异恶唑烷基]吡啶（SYP-Z048）对抑制各种子囊菌门和担子菌门的菌丝生长非常有效，其半数有效浓度（EC50）值在0.008至1.140微克/毫升之间。SYP-Z048对卵菌门生长的活性则弱得多，其EC50值>100微克/毫升。在针对桃褐腐病菌分离株的第二项体外试验中，SYP-Z048抑制了菌丝生长（EC50 = 0.013微克/毫升）、芽管伸长（EC50 = 0.007微克/毫升）和孢子形成，但不影响孢子萌发。在用桃褐腐病菌分离株接种的离体梨果实试验中，SYP-Z048表现出保护和治疗活性。田间试验表明，SYP-Z048是一种防治两个桃园中褐腐病的有效杀菌剂。当将其施用于复叶中一片番茄小叶上的单个部位时，SYP-Z048抑制了其余4片小叶上灰葡萄孢分离株的生长，这表明该杀菌剂在植物组织中有系统移动性。这些结果表明，SYP-Z048在管理由桃褐腐病菌引起的褐腐病以及由子囊菌门和担子菌门引起的其他病害方面具有潜力。
