Department of Thoracic Surgery, China Aerospace Science and Industry Corporation 731 Hospital, Peking, 100074, China.
Cell Biochem Biophys. 2023 Jun;81(2):325-336. doi: 10.1007/s12013-022-01121-0. Epub 2023 Jan 23.
Human papillomavirus (HPV) is a risk factor for lung cancer. However, the underlying mechanisms are not known. Long noncoding RNAs (lncRNAs) have been found to play an important role in the occurrence and development of lung cancer due to their particular characteristics. HPV-induced lung carcinogenesis is incompletely defined. We aimed to screen and clarify the functions of lncRNAs that are differentially expressed in HPV-related lung cancer. We found that lncRNA SNHG1 is upregulated in lung cancer cells infected with HPV16 E6 by qRT‒PCR. Further results demonstrated that SNHG1 overexpression facilitates the tube formation of human umbilical vein endothelial cells (HUVECs) in vitro. Our results also indicated that SNHG1 might function in lung cancer by binding with EGFR. Further studies revealed that SNHG1 overexpression could activate the nuclear factor κb (NF-κB) pathway, which increases the expression of interleukin-6 (IL-6). We also found that IL-6 can activate the STAT3 pathway, which promotes VEGF-D expression. These results expanded our understanding of SNHG1 as a new avenue for therapeutic intervention against lung cancer progression. Upregulation of SNHG1 by HPV infection might be an undefined link between lung cancer and HPV.
人乳头瘤病毒（HPV）是肺癌的一个风险因素。然而，其潜在机制尚不清楚。长链非编码 RNA（lncRNA）由于其特殊的特性，被发现与肺癌的发生和发展有重要关系。HPV 诱导的肺癌发生机制尚不完全明确。我们旨在筛选和阐明 HPV 相关肺癌中差异表达的 lncRNA 的功能。我们发现 qRT-PCR 结果显示，HPV16 E6 感染的肺癌细胞中 lncRNA SNHG1 表达上调。进一步的结果表明，SNHG1 的过表达促进了人脐静脉内皮细胞（HUVEC）的管腔形成。我们的研究结果还表明，SNHG1 可能通过与 EGFR 结合在肺癌中发挥作用。进一步的研究表明，SNHG1 的过表达可以激活核因子 κb（NF-κB）通路，从而增加白细胞介素 6（IL-6）的表达。我们还发现，IL-6 可以激活 STAT3 通路，促进 VEGF-D 的表达。这些结果扩展了我们对 SNHG1 的认识，将其作为治疗干预肺癌进展的新途径。HPV 感染上调 SNHG1 可能是肺癌和 HPV 之间未定义的联系。
