Qi Ao, Liu Lulu, Zhang Junjie, Chen Simei, Xu Simin, Chen Yusen, Zhang Lijiang, Cai Chun
Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, 524023, Guangdong, China.
The First DongGuan Affiliated Hospital of Guangdong Medical University, Dongguan, 523475, Guangdong, China.
Mol Neurobiol. 2023 May;60(5):2619-2631. doi: 10.1007/s12035-022-03157-y. Epub 2023 Jan 24.
Parkinson's disease (PD) is a progressive neurodegenerative disorder, characterized by high morbidity, high disability rate, and slow course of disease. The clinical diagnostic method of PD is complex and time-consuming, and there is no clear biomarker for clinical use. We aimed to investigate the plasma metabolites in PD and find out potential biomarkers with diagnostic ability. In the analysis of more than 40 metabolites including short-chain fatty acids, long-chain fatty acids, amino acids, and carbohydrates, the difference of short-chain fatty acids was observed. Acetic acid concentration was higher in PD than in healthy controls, and propanoic acid and 2,3,4-trihydroxybutyric acid were lower in PD. Compared with the early stage of PD, acetic acid increased significantly in the advanced stage of PD. Propanoic acid increased significantly in medicated PD compared with drug naïve PD. ROC analysis revealed acetic acid discriminated PD from healthy controls with 100% sensitivity, 88.9% specificity, and an area under the curve (AUC) of 0.981, and propanoic acid discriminated PD from healthy controls with an AUC of 0.981, 100% sensitivity, and 94.4% specificity. Acetic acid and propanoic acid may be a potential biomarker for differentiating PD from health, and the propanoic acid was evaluated as the most potential diagnostic marker because of its extremely high sensitivity and specificity.
帕金森病（PD）是一种进行性神经退行性疾病，其特点是发病率高、致残率高且病程缓慢。PD的临床诊断方法复杂且耗时，目前尚无明确的临床可用生物标志物。我们旨在研究PD患者的血浆代谢物，找出具有诊断能力的潜在生物标志物。在对包括短链脂肪酸、长链脂肪酸、氨基酸和碳水化合物在内的40多种代谢物进行分析时，观察到了短链脂肪酸的差异。PD患者的乙酸浓度高于健康对照，而丙酸和2,3,4-三羟基丁酸在PD患者中较低。与PD早期相比，PD晚期的乙酸显著增加。与未用药的PD患者相比，用药的PD患者丙酸显著增加。ROC分析显示，乙酸区分PD与健康对照的灵敏度为100%，特异度为88.9%，曲线下面积（AUC）为0.981；丙酸区分PD与健康对照的AUC为0.981，灵敏度为100%，特异度为94.4%。乙酸和丙酸可能是区分PD与健康状态的潜在生物标志物，丙酸因其极高的灵敏度和特异度被评估为最具潜力的诊断标志物。
