Siegers C P, Schenke M, Younes M
Institute of Toxicology, Medical University of Lübeck, Federal Republic of Germany.
J Toxicol Environ Health. 1987;22(2):141-8. doi: 10.1080/15287398709531058.
Sublethal doses of CdCl2 (3 mg/kg iv), HgCl2 (2 mg/kg iv), or NaVO3 (6 mg/kg iv) did not alter the content of reduced glutathione (GSH) in the livers of mice during the 24-h observation period. In the kidneys, a tendency to increased GSH content was seen, especially after HgCl2 treatment; in lung and brain the GSH levels were significantly lowered upon the treatment with all three metals. The activities of GSH S-transferase toward an aryl substrate (CDNB; 1-chloro-2,4-dinitrobenzene) was enhanced in all tissues by the administration of HgCl2 greater than NaVO3 greater than CdCl2. The activity of GSH S-transferase toward an epoxide substrate [1,2-epoxy-3-(p-nitrophenoxy)propane was only measurable in the livers and was inhibited 1 and 2 h after the administration of HgCl2 and NaVO3. It is concluded that sublethal doses of CdCl2, HgCl2, or NaVO3 do not impair the GSH concentration and GSH-conjugating enzyme activities toward the aryl substrate in different target organs of their toxicity, which is in contrast to results obtained in vitro.
在24小时观察期内，静脉注射致死剂量以下的氯化镉（3毫克/千克）、氯化汞（2毫克/千克）或钒酸钠（6毫克/千克）不会改变小鼠肝脏中还原型谷胱甘肽（GSH）的含量。在肾脏中，尤其是在氯化汞处理后，可见GSH含量有增加的趋势；在用这三种金属处理后，肺和脑中的GSH水平显著降低。通过给予氯化汞、钒酸钠和氯化镉，所有组织中谷胱甘肽S-转移酶对芳基底物（CDNB；1-氯-2,4-二硝基苯）的活性均增强，且氯化汞的增强作用大于钒酸钠，钒酸钠大于氯化镉。谷胱甘肽S-转移酶对环氧化物底物[1,2-环氧-3-（对硝基苯氧基）丙烷]的活性仅在肝脏中可检测到，在给予氯化汞和钒酸钠后1小时和2小时受到抑制。得出的结论是，致死剂量以下的氯化镉、氯化汞或钒酸钠不会损害其毒性不同靶器官中GSH的浓度以及GSH对芳基底物的结合酶活性，这与体外实验结果相反。
