淀粉摄入量、淀粉酶基因拷贝数变异、血浆蛋白与心血管疾病和死亡率风险。

Starch intake, amylase gene copy number variation, plasma proteins, and risk of cardiovascular disease and mortality.

机构信息

School of Public Health, Tianjin Medical University, 22 Qixiangtai Road, Heping District, Tianjin, 300070, China.

Nutritional Epidemiology, Department of Clinical Sciences in Malmö, Lund University, Malmö, Sweden.

出版信息

BMC Med. 2023 Jan 24;21(1):27. doi: 10.1186/s12916-022-02706-5.

DOI:10.1186/s12916-022-02706-5

PMID:36691017

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9872432/

Abstract

BACKGROUND

Salivary amylase, encoded by the AMY1 gene, initiate the digestion of starch. Whether starch intake or AMY1 copy number is related to disease risk is currently rather unknown. The aim was to investigate the association between starch intake and AMY1 copy number and risk of cardiovascular disease (CVD) and mortality and whether there is an interaction. In addition, we aim to identify CVD-related plasma proteins associated with starch intake and AMY1 copy number.

METHODS

This prospective cohort study used data from 21,268 participants from the Malmö Diet and Cancer Study. Dietary data were collected through a modified diet history method and incident CVD and mortality were ascertained through registers. AMY1 gene copy number was determined by droplet digital polymerase chain reaction, a risk score of 10 genetic variants in AMY1 was measured, and a total of 88 selected CVD-related proteins were measured. Cox proportional hazards regression was used to analyze the associations of starch intake and AMY1 copy number with disease risk. Linear regression was used to identify plasma proteins associated with starch intake and AMY1 copy number.

RESULTS

Over a median of 23 years' follow-up, 4443 individuals developed CVD event and 8125 died. After adjusting for potential confounders, a U-shape association between starch intake and risk of CVD (P-nonlinearity = 0.001) and all-cause mortality (P-nonlinearity = 0.03) was observed. No significant association was found between AMY1 copy number and risk of CVD and mortality, and there were no interactions between starch intake and AMY1 copy number (P interaction > 0.23). Among the 88 plasma proteins, adrenomedullin, interleukin-1 receptor antagonist protein, fatty acid-binding protein, leptin, and C-C motif chemokine 20 were associated with starch intake after adjusting for multiple testing.

CONCLUSIONS

In this large prospective study among Swedish adults, a U-shaped association between starch intake and risk of CVD and all-cause mortality was found. Several plasma proteins were identified which might provide information on potential pathways for such association. AMY1 copy number was not associated with CVD risk or any of the plasma proteins, and there was no interaction between starch intake and AMY1 copy number on disease risk.

摘要

背景

唾液淀粉酶由 AMY1 基因编码，它启动淀粉的消化。目前尚不清楚淀粉摄入量或 AMY1 拷贝数与疾病风险之间的关系。本研究旨在探讨淀粉摄入量与 AMY1 拷贝数与心血管疾病（CVD）风险和死亡率之间的关系，以及是否存在相互作用。此外，我们旨在确定与淀粉摄入量和 AMY1 拷贝数相关的与 CVD 相关的血浆蛋白。

方法

本前瞻性队列研究使用了来自马尔默饮食与癌症研究的 21268 名参与者的数据。通过改良饮食史法收集饮食数据，通过登记册确定 CVD 事件和死亡率。通过液滴数字聚合酶链反应确定 AMY1 基因拷贝数，测量 10 个 AMY1 遗传变异的风险评分，并测量了总共 88 种与 CVD 相关的选定蛋白。使用 Cox 比例风险回归分析淀粉摄入量和 AMY1 拷贝数与疾病风险的关系。使用线性回归来确定与淀粉摄入量和 AMY1 拷贝数相关的血浆蛋白。

结果

在中位数为 23 年的随访期间，有 4443 人发生 CVD 事件，8125 人死亡。在调整了潜在混杂因素后，观察到淀粉摄入量与 CVD 风险（P-非线性=0.001）和全因死亡率（P-非线性=0.03）之间呈 U 形关联。AMY1 拷贝数与 CVD 和死亡率风险之间没有显著关联，并且淀粉摄入量和 AMY1 拷贝数之间没有相互作用（P 交互作用>0.23）。在 88 种血浆蛋白中，肾上腺髓质素、白细胞介素-1 受体拮抗剂蛋白、脂肪酸结合蛋白、瘦素和 C-C 基序趋化因子 20 在经过多次测试调整后与淀粉摄入量相关。