A-激酶锚定蛋白 95 参与结直肠癌中 ERK1/2-Elk-1 信号转导。

A-Kinase Anchor Protein 95 Is Involved in ERK1/2-Elk-1 Signal Transduction in Colon Cancer.

机构信息

Center of Gallbladder Disease, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200092, China.

Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, China.

出版信息

Anal Cell Pathol (Amst). 2023 Jan 14;2023:8242646. doi: 10.1155/2023/8242646. eCollection 2023.

DOI:10.1155/2023/8242646

PMID:36691407

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9867590/

Abstract

OBJECTIVES

To assess A-kinase anchor protein 95 (AKAP95), B-Raf, extracellular regulated protein kinases 1/2 (ERK1/2), and Elk-1 expression in colon cancer tissue, and characterize AKAP95 associations with B-Raf, ERK1/2, Elk-1, and colon cancer clinicopathological indices.

METHODS

The immunohistochemistry streptavidin-perosidase (SP) method was used to determine protein expression levels in 64 colon cancer and 32 para-carcinoma tissue specimens.

RESULTS

(1) Positive AKAP95 expression rates in colon cancer tissue were higher when compared with para-carcinoma tissue (92.19% vs. 59.38%, < 0.05). Similar findings were determined for B-Raf (76.56% vs. 25%, < 0.05), ERK1/2 (90.63% vs. 31.25%, < 0.05), and Elk-1 levels (92.19% vs. 40.63%, < 0.05). (2) No significant associations were identified between AKAP95, B-Raf, ERK1/2, and Elk-1 protein expression and degree of differentiation, histological type, and lymph node metastasis in colon cancer samples ( > 0.05); however, in The Cancer Genome Atlas and Gene Expression Omnibus datasets, AKAP95 was closely related to immune infiltration, and highly expressed AKAP95 was negatively associated with overall survival and relapse free survival rates in colon cancer patients. (3) Correlations were observed between AKAP95 and ERK1/2, AKAP95 and Elk-1, B-Raf and ERK1/2, B-Raf and Elk-1, and ERK1/2 and Elk-1 (all < 0.05), but no correlation was observed between AKAP95 and B-Raf ( > 0.05).

CONCLUSIONS

AKAP95 may affect immune infiltration levels in colon cancer by participating in ERK1/2-Elk-1 signal transduction.

摘要

目的

评估 AKAP95、B-Raf、细胞外调节蛋白激酶 1/2（ERK1/2）和 Elk-1 在结肠癌组织中的表达，并分析 AKAP95 与 B-Raf、ERK1/2、Elk-1 以及结肠癌临床病理指标的关系。

方法

采用免疫组织化学链霉亲和素-过氧化物酶（SP）法检测 64 例结肠癌和 32 例癌旁组织标本中的蛋白表达水平。

结果

（1）结肠癌组织中 AKAP95 的阳性表达率高于癌旁组织（92.19% vs. 59.38%， < 0.05），B-Raf（76.56% vs. 25%， < 0.05）、ERK1/2（90.63% vs. 31.25%， < 0.05）和 Elk-1 水平（92.19% vs. 40.63%， < 0.05）也存在类似的结果。（2）在结肠癌样本中，AKAP95、B-Raf、ERK1/2 和 Elk-1 蛋白表达与分化程度、组织学类型和淋巴结转移之间无显著相关性（ > 0.05）；然而，在 The Cancer Genome Atlas 和 Gene Expression Omnibus 数据集，AKAP95 与免疫浸润密切相关，高表达的 AKAP95 与结肠癌患者的总生存率和无复发生存率呈负相关。（3）AKAP95 与 ERK1/2、AKAP95 与 Elk-1、B-Raf 与 ERK1/2、B-Raf 与 Elk-1、ERK1/2 与 Elk-1 之间均存在相关性（均 < 0.05），但 AKAP95 与 B-Raf 之间无相关性（ > 0.05）。