Liu Wenzhi, Hua Suhang, Dai Yue, Yuan Yangyang, Yang Jinghui, Deng Jiali, Huo Yunjie, Chen Xiaoxuan, Teng Bogang, Yu Xiuyi, Zhang Yongxing
Department of Gastrointestinal Surgery, Affiliated Zhongshan Hospital of Dalian University Dalian 116001, PR China.
School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University Shenyang 110016, Liaoning, PR China.
Int J Clin Exp Pathol. 2015 Nov 1;8(11):14315-24. eCollection 2015.
The purpose of this study was to investigate the expression of A-kinase anchor protein 95 (AKAP95), cell cycle protein E1 (cyclinE1) and D1 (cyclinD1), and gap junction protein connexin 43 (Cx43) in ovarian cancer tissues, the relationship between four proteins and clinicopathologic parameters, and the correlation between these proteins.
The expression of proteins in 54 cases of ovarian cancer tissues was detected by immunohistochemical method.
The positive expression rates of AKAP95, cyclinD1 and cyclinE1 in ovarian cancer tissues were 72.22%, 66.67% and 79.63%, respectively, which were higher than that of ovarian pericarcinoma tissues expressing as 33.33%, 25% and 8.30% (P<0.05). The positive expression rate of Cx43 in ovarian cancer tissues was 40.74%, which was lower than that of ovarian pericarcinoma tissues expressing as 75%; respectively, and the difference was statistically significant between groups (P<0.05). The expression of cyclinD1 in ovarian cancer tissues was related to the histologic type (P<0.05) while it showed no correlation with the degree of differentiation (P>0.05). Additionally, the expression of AKAP95, Cx43 and cyclinE1 in ovarian cancer tissues showed no correlation with the degree of differentiation or the histologic type (P>0.05). Protein expressions of AKAP95, Cx43 and cyclinE1 were correlated with each other (P<0.05), and the expressions of cyclinD1, cyclinE1 and Cx43 were also correlated with each other (P<0.05). However, AKAP95 and cyclinD1 showed no correlation (P>0.05).
AKAP95, cyclinD1 and cyclinE1 play an important role in promoting the process of ovarian cancer formation. The tumor inhibitory effects of Cx43 protein on the pathogenesis of ovarian cancer were weakened. The expression of cyclinD1 in ovarian cancer tissues is related to the histologic type while it shows no correlation with the degree of differentiation. Additionally, the expression of AKAP95, Cx43 and cyclinE1 in ovarian cancer tissues shows no correlation with the degree of differentiation or the histologic type. AKAP95 expression is correlated with Cx43 and cyclinE1 expression; Cx43 expression is correlated with AKAP95, cyclinD1 and cyclinE1 expression; cyclinE1 expression is correlated with AKAP95, Cx43, cyclinD1 expression; cyclinD1 expression is correlated with Cx43 and cyclinE1 expression, while AKAP95 and cyclinD1 show no correlation.
本研究旨在探讨A激酶锚定蛋白95（AKAP95）、细胞周期蛋白E1（cyclinE1）和D1（cyclinD1）以及缝隙连接蛋白连接蛋白43（Cx43）在卵巢癌组织中的表达情况、这四种蛋白与临床病理参数之间的关系以及这些蛋白之间的相关性。
采用免疫组织化学方法检测54例卵巢癌组织中蛋白的表达。
卵巢癌组织中AKAP95、cyclinD1和cyclinE1的阳性表达率分别为72.22%、66.67%和79.63%，高于卵巢癌旁组织的表达率，分别为33.33%、25%和8.30%（P<0.05）。卵巢癌组织中Cx43的阳性表达率为40.74%，低于卵巢癌旁组织的表达率75%，组间差异有统计学意义（P<0.05）。卵巢癌组织中cyclinD1的表达与组织学类型有关（P<0.05），而与分化程度无关（P>0.05）。此外，卵巢癌组织中AKAP95、Cx43和cyclinE1的表达与分化程度或组织学类型无关（P>0.05）。AKAP95、Cx43和cyclinE1的蛋白表达相互相关（P<0.05），cyclinD1、cyclinE1和Cx43的表达也相互相关（P<0.05）。然而，AKAP95和cyclinD1无相关性（P>0.05）。
AKAP95、cyclinD1和cyclinE1在促进卵巢癌形成过程中起重要作用。Cx43蛋白对卵巢癌发病机制的肿瘤抑制作用减弱。卵巢癌组织中cyclinD1的表达与组织学类型有关，而与分化程度无关。此外，卵巢癌组织中AKAP95、Cx43和cyclinE1的表达与分化程度或组织学类型无关。AKAP95表达与Cx43和cyclinE1表达相关；Cx43表达与AKAP95、cyclinD1和cyclinE1表达相关；cyclinE1表达与AKAP95、Cx43、cyclinD1表达相关；cyclinD1表达与Cx43和cyclinE1表达相关，而AKAP95和cyclinD1无相关性。
