Lee Jeong Hyun, Lee Ko Eun, Kang Sang Wook, Park Seung Hwan, Chae Yong Kwon, Lee Myoung-Han, Kweon Dong-Keun, Choi Sung Chul, Nam Ok Hyung
Department of Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea.
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, South Korea.
Oral Dis. 2024 Mar;30(2):518-527. doi: 10.1111/odi.14517. Epub 2023 Feb 5.
The objective of the study was to evaluate the healing effect of hyaluronic acid films on palatal wounds.
After making 5-mm diameter palatal wounds, 72 rats were randomly assigned to three groups: control, hyaluronic acid gel, and hyaluronic acid film. The animals were sacrificed at 3, 7, and 21 days after the experiment. Clinical, histological, and RT-PCR analyses were performed. Human ex vivo oral mucosa models were used. Histological analysis and pan-cytokeratin staining were performed at 5 days after wound creation.
In rat model, both gels and films showed favorable healing on Days 3 and 7 compared with healing in the control (p < 0.05). Film showed remarkable VEGF and α-SMA expression than did the others (p < 0.05). Immunohistochemical analysis showed that film exhibited significantly lower CD68 and greater α-SMA and vimentin expression levels than those in the others (p < 0.05). In human model, re-epithelialization rate of film group was significantly higher than that of the others. Complete epithelial regeneration was confirmed only in film group using pan-cytokeratin staining.
Within the limits of this study, hyaluronic acid film outperformed gels in terms of palatal wound healing in both models.
本研究的目的是评估透明质酸膜对腭部伤口的愈合效果。
在制作直径为5毫米的腭部伤口后，将72只大鼠随机分为三组：对照组、透明质酸凝胶组和透明质酸膜组。在实验后第3、7和21天处死动物。进行临床、组织学和逆转录聚合酶链反应分析。使用人离体口腔黏膜模型。在伤口形成后第5天进行组织学分析和全细胞角蛋白染色。
在大鼠模型中，与对照组相比，凝胶和膜在第3天和第7天均显示出良好的愈合效果（p<0.05）。膜显示出比其他组更显著的血管内皮生长因子（VEGF）和α-平滑肌肌动蛋白（α-SMA）表达（p<0.05）。免疫组织化学分析表明，膜的CD68表达显著低于其他组，而α-SMA和波形蛋白表达水平高于其他组（p<0.05）。在人体模型中，膜组的再上皮化率显著高于其他组。仅在膜组使用全细胞角蛋白染色证实了完全上皮再生。
在本研究的范围内，在两个模型的腭部伤口愈合方面，透明质酸膜优于凝胶。
