Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
National Risk Assessment Laboratory for Antimicrobial Resistant of Microorganisms in Animals, South China Agricultural University, Guangzhou 510642, China.
J Antimicrob Chemother. 2023 Mar 2;78(3):802-809. doi: 10.1093/jac/dkad014.
To investigate the epidemiology of fosB-positive Staphylococcus aureus in waterfowl farms in the Pearl River tributaries in Guangdong Province, China in 2020.
A total of 63 S. aureus were recovered from 315 samples collected from six duck farms and one goose farm. PFGE, WGS and analysis were performed on 19 fosB-positive S. aureus.
The fosfomycin resistance rate of the strains was as high as 52.4% (33/63), and 30.1% (19/63) of the strains carried fosB. Resistance gene prediction results showed that duck farm environment-derived strains contained the oxazolidinone drug resistance gene optrA. All fosB-positive S. aureus were MRSA and most of them were MDR, mainly ST9-t899 and ST164-t899. PFGE showed that fosB-positive S. aureus from humans and ducks could be clustered into the same clade. In addition, core-genome SNP analysis showed that clonal transmission of S. aureus occurred between humans and water. Pan-genome analysis showed that S. aureus had an open pangenome. The fosB gene was located on 2610-2615 bp plasmids, which all contained a broad host-range plasmid replication protein family 13. Small plasmids carrying the fosB gene could be found in different multilocus STs of S. aureus.
This study indicated that duck farms in Guangdong, China could be an important reservoir of fosB-positive S. aureus. The spread of drug-resistant bacteria in waterfowl farms requires further monitoring.
调查 2020 年中国广东省珠江支流水禽养殖场中耐福西林阳性金黄色葡萄球菌的流行病学情况。
从 6 个鸭场和 1 个鹅场采集的 315 个样本中共分离出 63 株金黄色葡萄球菌。对 19 株耐福西林阳性金黄色葡萄球菌进行 PFGE、WGS 分析。
菌株的磷霉素耐药率高达 52.4%（33/63），30.1%（19/63）的菌株携带 fosB。耐药基因预测结果显示，鸭场环境来源的菌株含有唑烷酮类药物耐药基因 optrA。所有耐福西林阳性金黄色葡萄球菌均为 MRSA，且大多为 MDR，主要为 ST9-t899 和 ST164-t899。PFGE 显示，来自人和鸭的耐福西林阳性金黄色葡萄球菌可聚类到同一分支。此外，核心基因组 SNP 分析表明，金黄色葡萄球菌在人与水之间存在克隆传播。泛基因组分析表明，金黄色葡萄球菌具有开放的泛基因组。fosB 基因位于 2610-2615bp 质粒上，均含有广泛宿主范围质粒复制蛋白家族 13。可在不同的金黄色葡萄球菌多位点 ST 中发现携带 fosB 基因的小质粒。
本研究表明，中国广东的鸭场可能是耐福西林阳性金黄色葡萄球菌的重要储存库。水禽养殖场中耐药菌的传播需要进一步监测。
