VISAFELab, Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Avda. Catedrático Agustín Escardino 7, Valencia, Paterna 46980, Spain.
Department of Food Science and Technology, CEBAS-CSIC, Research Group on Quality and Safety of Fruits and Vegetables, Campus Universitario de Espinardo, 25, Murcia 30100, Spain.
Water Res. 2023 Mar 1;231:119621. doi: 10.1016/j.watres.2023.119621. Epub 2023 Jan 18.
Besides nasopharyngeal swabs, monkeypox virus (MPXV) DNA has been detected in a variety of samples such as saliva, semen, urine and fecal samples. Using the environmental surveillance network previously developed in Spain for the routine wastewater surveillance of SARS-CoV-2 (VATar COVID-19), we have analyzed the presence of MPXV DNA in wastewater from different areas of Spain. Samples (n = 312) from 24 different wastewater treatment plants were obtained between May 9 (week 19 of 2022) and August 4 (week 31 of 2022). Following concentration of viral particles by a validated aluminum adsorption-precipitation method, a qPCR procedure allowed us to detect MPXV DNA in 56 wastewater samples collected from May 16 to August 4, 2022, with values ranging between 2.2 × 10 to 8.7 × 10 genome copies (gc)/L. This study shows that MPXV DNA can be reproducibly detected by qPCR in longitudinal samples collected from different Spanish wastewater treatment plants. According to data from the National Epidemiological Surveillance Network (RENAVE) in Spain a total of 6,119 cases have been confirmed as of August 19, 2022. However, and based on the wastewater data, the reported clinical cases seem to be underestimated and asymptomatic infections may be more frequent than expected.
除了鼻咽拭子外，猴痘病毒（MPXV）的 DNA 也已在唾液、精液、尿液和粪便等各种样本中被检测到。我们利用西班牙先前为 SARS-CoV-2（VATar COVID-19）的常规废水监测而开发的环境监测网络，分析了西班牙不同地区废水中 MPXV DNA 的存在情况。在 2022 年 5 月 9 日（第 19 周）至 8 月 4 日（第 31 周）期间，从 24 个不同的污水处理厂获得了 312 个样本。通过经过验证的铝吸附沉淀法浓缩病毒颗粒后，qPCR 程序可在 2022 年 5 月 16 日至 8 月 4 日采集的 56 个废水中检测到 MPXV DNA，其值范围在 2.2×10 到 8.7×10 基因组拷贝（gc）/L 之间。这项研究表明，qPCR 可在来自不同西班牙污水处理厂的纵向样本中重复检测到 MPXV DNA。根据西班牙国家流行病学监测网络（RENAVE）的数据，截至 2022 年 8 月 19 日，已确认的病例总数为 6119 例。然而，根据废水数据，报告的临床病例似乎被低估了，无症状感染可能比预期更常见。
