Dodangeh Masood, Farrokhpour Hossein, Ghaziaskar Hassan S, Tabrizchi Mahmoud, Momeni Mohamad Mohsen, Motalebian Majid
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
J Am Soc Mass Spectrom. 2023 Mar 1;34(3):374-382. doi: 10.1021/jasms.2c00246. Epub 2023 Jan 24.
Substrate-assisted laser desorption/ionization (SALDI) is a kind of soft ionization method that is most suitable for the analysis of low molecular weight analytes when it is coupled with a time-of-flight mass spectrometer. Unlike the conventional matrix-assisted laser desorption/ionization, there is no interference in the SALDI with matrices for the low mass analyte peaks (/ < 700). The focus of this work is to develop substrates based on nanomaterials to obtain higher sensitivity, better reproducibility, and easier preparation. The mass spectra of some small molecules (capecitabine, hemin, methadone, noscapine, oxycodone, thebaine, malathion, chlorpyrifos, ethion, permethrin, and phosalone) deposited on the TiO-nanotube (TiO-NTs) plate by the SALDI-TOF-MS technique are reported. The nanotubes are synthesized in different diameter sizes of nanotubes via the anodizing method. The intensity of the analyte peaks and the softness of ionization are optimized by varying the diameter of nanotubes and adding relevant alkali salts to the analytes. In addition, the reproducibility of the signal intensity of analytes is optimized by changing the surface hydrophilicity of the TiO-NT plate.
基质辅助激光解吸/电离（SALDI）是一种软电离方法，当与飞行时间质谱仪联用时，它最适合分析低分子量分析物。与传统的基质辅助激光解吸/电离不同，SALDI对低质量分析物峰（/<700）的基质没有干扰。这项工作的重点是开发基于纳米材料的基质，以获得更高的灵敏度、更好的重现性和更简便的制备方法。报道了通过SALDI-TOF-MS技术沉积在TiO纳米管（TiO-NTs）板上的一些小分子（卡培他滨、血红素、美沙酮、那可丁、羟考酮、蒂巴因、马拉硫磷、毒死蜱、乙硫磷、氯菊酯和伏杀硫磷）的质谱。通过阳极氧化法合成了不同管径尺寸的纳米管。通过改变纳米管的直径并向分析物中添加相关碱金属盐来优化分析物峰的强度和电离的软度。此外，通过改变TiO-NT板的表面亲水性来优化分析物信号强度的重现性。
