Arakawa Ryuichi, Kawasaki Hideya
Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita, Osaka, Japan.
Anal Sci. 2010;26(12):1229-40. doi: 10.2116/analsci.26.1229.
Surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF-MS) using nanoparticles (NPs) and nanostructured surfaces as the LDI-assisting nanomaterials is a soft ionization technique that features minimal fragmentation of analytes. As compared to traditional matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) using organic matrices, SALDI-MS affords several advantages, such as the ability to detect small molecules (<500 Da), easy sample preparation, low-noise background, high salt tolerance, and fast data collection without the use of an organic matrix. The performance of SALDI has been further improved recently in terms of the detection sensitivity, detection mass range from the low- to the high-mass region, a soft LDI process, the detection of both polar and nonpolar compounds, the selective detection of analytes from a complex mixture using functionalized NPs, and various applications, including imaging mass spectrometry. This review summarizes recent developments pertaining to various NPs and nanostructured surfaces for SALDI-MS.
使用纳米颗粒（NPs）和纳米结构表面作为激光解吸电离辅助纳米材料的表面辅助激光解吸/电离飞行时间质谱（SALDI-TOF-MS）是一种软电离技术，其特点是分析物的碎片化程度最小。与使用有机基质的传统基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）相比，SALDI-MS具有多个优点，例如能够检测小分子（<500 Da）、样品制备简单、背景噪声低、耐盐性高以及无需使用有机基质即可快速收集数据。最近，SALDI在检测灵敏度、从低质量到高质量区域的检测质量范围、软激光解吸电离过程、极性和非极性化合物的检测、使用功能化纳米颗粒从复杂混合物中选择性检测分析物以及包括成像质谱在内的各种应用方面的性能得到了进一步提高。本综述总结了SALDI-MS在各种纳米颗粒和纳米结构表面方面的最新进展。
