Dkhil Mohamed A, Al-Quraishy Saleh, Wahab Rizwan
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia ; Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Int J Nanomedicine. 2015 Mar 11;10:1961-8. doi: 10.2147/IJN.S79944. eCollection 2015.
Nanomedicine has recently emerged as a better option for the treatment of various diseases. Here, we investigated the in vivo anticoccidial properties of zinc oxide nanoparticles (ZNPs). ZNPs were crystalline in nature, with a smooth and spherical surface and a diameter in the range of ~10-15 nm. The X-ray diffraction pattern was utilized to identify the crystalline property of the grown ZNPs, whereas field emission scanning electron microscopy was employed to check the size and morphology of the ZNPs. The data showed that mice infected with Eimeria papillata produced 29.7×10(3)±1,500 oocysts/g feces on day 5 postinfection. This output was significantly decreased, to 12.5×10(3)±1,000 oocysts, in mice treated with ZNPs. Infection also induced inflammation and injury of the jejunum. This was evidenced (1) through an increase in the inflammatory histological score, (2) through increased production of nitric oxide and malondialdehyde, and (3) through a decrease in both the glutathione level and goblet cell number in mice jejuna. All these infection-induced parameters were significantly altered during treatment with ZNPs. Our results indicate, therefore, that ZNPs have protective effects against E. papillata-induced coccidiosis.
纳米医学最近已成为治疗各种疾病的更好选择。在此，我们研究了氧化锌纳米颗粒（ZNPs）的体内抗球虫特性。ZNPs本质上是晶体，具有光滑的球形表面，直径在~10 - 15纳米范围内。利用X射线衍射图谱来确定生长的ZNPs的晶体特性，而用场发射扫描电子显微镜来检查ZNPs的大小和形态。数据显示，感染乳头艾美耳球虫的小鼠在感染后第5天产生29.7×10³±1500个卵囊/克粪便。在用ZNPs治疗的小鼠中，这一产量显著降低至12.5×10³±1000个卵囊。感染还诱导空肠炎症和损伤。这通过以下几点得到证实：（1）炎症组织学评分增加；（2）一氧化氮和丙二醛产量增加；（3）小鼠空肠中谷胱甘肽水平和杯状细胞数量减少。在用ZNPs治疗期间，所有这些感染诱导的参数都发生了显著变化。因此，我们的结果表明，ZNPs对乳头艾美耳球虫引起的球虫病具有保护作用。
