Maodaa Saleh, Al-Shaebi Esam M, Abdel-Gaber Rewaida, Alatawi Afaf, Alawwad Sarah, Alhomoud Dalal, Al-Quraishy Saleh
Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Department of Food Science and Nutrition, College of Food and Agricultural Science, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
Vet Sci. 2024 Jul 14;11(7):314. doi: 10.3390/vetsci11070314.
spp. are responsible for the economic loss of both domestic and wild animals due to coccidiosis, the most common parasitic disease. The resistance to currently available drugs used to treat coccidiosis has been proven. Medicinal plants that contain physiologically active phytochemicals have been widely used in traditional medicine. leaf extract (TPLE) has been shown to exhibit pharmacological, antioxidant, and anticoccidial properties in different experiments. Here, our investigation focused on how leaf extract affected the way that caused intestinal injury in mice. Thirty-five male Swiss albino mice were divided into seven groups, as follows: group I: untreated and uninfected (negative control); group II: uninfected, treated group with TPLE (150 mg/kg b.w); and group III: infected untreated (positive control). Groups III-VII were orally administered 10 sporulated oocysts. A total of 60 min after infection, groups IV-VI were treated for five successive days with 50, 150, and 250 mg/kg b.w TPLE, respectively, while group VII was treated with amprolium (120 mg/kg b.w.). The mice had been euthanized on the fifth day post-infection, and the jejunum tissues were prepared for histology and oxidative stress studies. A total of 150 mg/kg of TPLE was the most effective dosage, significantly decreasing oocyst output by about 80.5%, accompanied by a significant reduction in the number of developmental parasitic phases in jejunal sections. In addition, the decrease in the number of goblet cells in the jejuna of mice raised after treatment. Also, TPLE greatly diminished the body weight loss of infected mice. Moreover, our research proved that TPLE reduced oxidative damage due to infection via decreasing intestinal malondialdehyde (MDA) and nitric oxide (NO) levels and increasing reduced superoxide dismutase (SOD) and glutathione (GSH) levels. These results demonstrated that TPLE had potent anticoccidial properties. TPE's efficacy as a natural antioxidant has also been demonstrated in reducing oxidative stress and enhancing antioxidant systems to mitigate biochemical and histological changes in the jejunum caused by .
某些物种（spp.）因球虫病这种最常见的寄生虫病，导致家畜和野生动物出现经济损失。已证实对目前用于治疗球虫病的药物产生了耐药性。含有生理活性植物化学物质的药用植物已在传统医学中广泛使用。[植物名称]叶提取物（TPLE）在不同实验中已显示出具有药理、抗氧化和抗球虫特性。在此，我们的研究聚焦于[植物名称]叶提取物如何影响[寄生虫名称]在小鼠中造成肠道损伤的方式。35只雄性瑞士白化小鼠被分为七组，如下：第一组：未处理且未感染（阴性对照）；第二组：未感染，用TPLE（150毫克/千克体重）处理的组；第三组：感染但未处理（阳性对照）。第三至七组经口给予10个孢子化的[寄生虫名称]卵囊。感染后共60分钟，第四至六组分别用50、150和250毫克/千克体重的TPLE连续处理五天，而第七组用氨丙啉（120毫克/千克体重）处理。在感染后第五天对小鼠实施安乐死，并制备空肠组织用于组织学和氧化应激研究。150毫克/千克体重的TPLE是最有效的剂量，显著降低卵囊产量约80.5%，同时空肠切片中发育中的寄生虫阶段数量显著减少。此外，处理后小鼠空肠中杯状细胞数量减少。而且，TPLE极大地减少了感染小鼠的体重减轻。此外，我们的研究证明TPLE通过降低肠道丙二醛（MDA）和一氧化氮（NO）水平以及提高还原型超氧化物歧化酶（SOD）和谷胱甘肽（GSH）水平，减少了因[寄生虫名称]感染引起的氧化损伤。这些结果表明TPLE具有强大的抗球虫特性。TPE作为一种天然抗氧化剂在减轻[寄生虫名称]引起的空肠生化和组织学变化方面，通过降低氧化应激和增强抗氧化系统的功效也得到了证实。
