Akkas Onder, Uslu Hakan, Yilmaz Isa, Aydın Yener, Korkut Ercan, Yilmaz Ahmet
Department of Medical Microbiology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
Iran J Parasitol. 2022 Oct-Dec;17(4):554-561. doi: 10.18502/ijpa.v17i4.11283.
We aimed to determine the prevalence of Alveolar echinococcusis using ELISA in our region, to perform the specific differentiation of species by using the Western Blott method, to diagnose and treat this disease effectively in early periods, and to inform the patients about the results quickly.
From the provinces of Erzurum, Kars, Ardahan, Iğdır, Ağrı and Erzincan in the Eastern Anatolia Region, Turkey in August-September 2017, blood samples were provided from 305 participants (volunteer patients and their relatives), including 151 females (49.50%), whose ages ranged between 6-85 yr and were diagnosed as Alveolar echinococcosis (AE) and operated in concerned clinics.
EIgG ELISA was determined as positive in 29 (9.5%) participants, including 17 (11.3%) females and 12 (7.8%) males. In addition, Em2-Em18 ELISA was also determined as positive in 15 (4.9%) participants, including 9 (6%) females and 6 (3.9%) males. Tweleve (3.94 %) of these findings were observed as positive in terms of both tests. Through the verification done by Anti-EWB IgG, was identified in 7 (21.9%) of the female participants, in 1 (3.1%), and both parasites in 2 (6.2%). For male participants, EM was determined in 3 (9.3%) of them, EG in 1 (3.1%), and both parasites in 1 (3.1%).
AE continues to threaten public health in the region. In families where AE is detected positive, the reason for disease is based on nutrition; thus, it will be proper to check up all family members in the terms of infection.
我们旨在通过酶联免疫吸附测定（ELISA）确定我们地区肺泡型棘球蚴病的患病率，使用蛋白质印迹法进行物种特异性鉴别，在疾病早期有效诊断和治疗该疾病，并迅速将结果告知患者。
2017年8月至9月，从土耳其东安纳托利亚地区的埃尔祖鲁姆、卡尔斯、阿尔达汉、伊兹迪尔、阿格里和埃尔津詹省，采集了305名参与者（志愿患者及其亲属）的血样，其中包括151名女性（49.50%），年龄在6至85岁之间，被诊断为肺泡型棘球蚴病（AE）并在相关诊所接受手术。
酶联免疫吸附测定（EIgG ELISA）在29名（9.5%）参与者中被确定为阳性，其中包括17名（11.3%）女性和12名（7.8%）男性。此外，Em2-Em18 ELISA在15名（4.9%）参与者中也被确定为阳性，其中包括9名（6%）女性和6名（3.9%）男性。这些结果中有12名（3.94%）在两项检测中均为阳性。通过抗EWB IgG进行的验证，在7名（21.9%）女性参与者中鉴定出了 ，在1名（3.1%）中鉴定出了 ，在2名（6.2%）中鉴定出了两种寄生虫。对于男性参与者，在3名（9.3%）中确定有EM，在1名（3.1%）中确定有EG，在1名（3.1%）中确定有两种寄生虫。
AE继续威胁该地区的公共卫生。在检测出AE呈阳性的家庭中，疾病原因基于营养；因此，对所有家庭成员进行感染方面的检查是合适的。
