Guo Yue, Zhang Hui, Dong Haiyan, Lu Ming Ke, Li Xiaoyu
School of Medicine, Huzhou University, Huzhou, Zhejiang, China.
Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, Zhejiang, China.
Iran J Parasitol. 2022 Oct-Dec;17(4):589-595. doi: 10.18502/ijpa.v17i4.11287.
To explore the transcriptome profiling of the fourth-stage larvae of .
Two groups of fourth-stage larvae were collected to extract total RNA in Zhejiang, China 2020. Then, mRNA was separated and reverse transcribed into cDNA. Next-generation sequencing was used to explore the transcriptome information. Finally, to obtain the biological annotation information, the transcriptome information was run against the related databases, including Nr, GO, COG, KOG and ORF.
Overall, 128667 unigenes and 193059 transcripts were obtained. The Nr annotations of unigenes and transcripts showed that was the 5th and 4th most related species, respectively. Meanwhile, the annotation of unigenes and transcripts by querying GO, COG, KOG and ORF showed that L4 was extremely active in gene expression, concerning signal transduction, transcription, posttranslational modification, metabolism, etc.
The fourth-stage larvae of have their own profiling in the transcriptome, which is related to signal transduction, transcription, posttranslational modification, metabolism, etc.
探索[具体物种名称]第四期幼虫的转录组图谱。
2020年在中国浙江收集两组第四期幼虫以提取总RNA。然后，分离mRNA并逆转录成cDNA。使用新一代测序技术探索转录组信息。最后，为获得生物学注释信息，将转录组信息与包括Nr、GO、COG、KOG和ORF在内的相关数据库进行比对。
总体而言，获得了128667个单基因和193059个转录本。单基因和转录本的Nr注释表明，[具体物种名称]分别是第五和第四最相关物种。同时，通过查询GO、COG、KOG和ORF对单基因和转录本的注释表明，第四期幼虫在基因表达方面极其活跃，涉及信号转导、转录、翻译后修饰、代谢等。
[具体物种名称]的第四期幼虫在转录组方面有其自身图谱，这与信号转导、转录、翻译后修饰、代谢等有关。
