Pestechian Nader, Mohammadi Manesh Reza, Tavakoli Sanaz, Mokarian Fariborz, Rahmani Maryam
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Iran J Parasitol. 2022 Oct-Dec;17(4):497-505. doi: 10.18502/ijpa.v17i4.11277.
spp. are protozoan parasites that cause diarrhea in humans and animals. Subtyping data about spp. in Isfahan, Iran is limited; therefore, we aimed to study the prevalence rate of spp. in cancer patients, associated risk factors, and subtypes of spp.
Fecal samples were collected from 187 cancer patients from the Oncology Department of Seyed-al-Shohada Hospital, Isfahan University of Medical Sciences during 2014-2020 and screened for spp. using microscopical techniques. Nested PCR amplifying 18S rRNA gene was used to detect spp. in samples, followed by subtyping using nested PCR amplifying gp60 sequences.
Overall, the rate of infection with spp. was 4.3% (n=8). Five samples out of eight samples were identified as spp. using a nested PCR for the 18S rRNA gene, two subtypes of named IIaA18G3R1 (n = 2) and IIaA17G2R1 (n = 2), and one subtype of named IbA6G3 were identified by sequencing of the gp60. The IbA6G3 subtype has rarely been detected in other investigations.
This is the first survey on the subtyping of spp. in this region. The results of the present survey show both zoonotic and anthroponotic transmission routes in the region.
属原虫寄生虫，可导致人类和动物腹泻。伊朗伊斯法罕关于 属的亚型数据有限；因此，我们旨在研究癌症患者中 属的患病率、相关危险因素以及 属的亚型。
在2014 - 2020年期间，从伊斯法罕医科大学赛义德 - 阿尔 - 肖哈达医院肿瘤科的187名癌症患者中采集粪便样本，并使用显微镜技术对 属进行筛查。使用巢式PCR扩增18S rRNA基因来检测样本中的 属，随后使用巢式PCR扩增gp60序列进行亚型分析。
总体而言， 属的感染率为4.3%（n = 8）。在八个样本中，有五个样本通过18S rRNA基因的巢式PCR鉴定为 属，通过gp60测序鉴定出两种 亚型，即IIaA18G3R1（n = 2）和IIaA17G2R1（n = 2），以及一种 亚型，即IbA6G3。IbA6G3亚型在其他调查中很少被检测到。
这是该地区首次对 属进行亚型分析的调查。本次调查结果显示该地区存在人畜共患病和人传人传播途径。
