Uema Masashi, Yonemitsu Kenzo, Sasaki Yoshimasa, Asakura Hiroshi
National Institute of Health Sciences, 3-25-26, Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
Murayama Brunch, National Institute of Infectious Diseases, 4-7-1, Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
AIMS Microbiol. 2022 Dec 26;8(4):566-574. doi: 10.3934/microbiol.2022036. eCollection 2022.
Hepatitis E virus (HEV) is a zoonotic pathogen that circulates mainly between pigs and humans. In Japan, the number of confirmed HEV cases has increased over the past decade, with the majority reported as domestic HEV infections. HEV-infected pork products may be associated with this increase, but there is limited information on HEV in pork in Japanese markets. From February to March 2020, gallbladders were collected from 200 slaughtered pigs shipped from 14 farms and were surveyed to detect HEV RNA in bile using reverse transcription quantitative polymerase chain reaction. The samples were then sequenced and genotyped. Twenty pigs were positive for HEV ribonucleic acid, and seven samples had Ct values of less than 30. Among these 20 pigs, virus strains from 14 pigs were determined as genotype 3. This report indicated that HEV-contaminated pork liver was shipped to consumer markets and demonstrated the importance of detection of HEV in meat ready for shipment.
戊型肝炎病毒（HEV）是一种主要在猪和人之间传播的人畜共患病原体。在日本，过去十年中戊型肝炎确诊病例数量有所增加，大多数报告为国内戊型肝炎感染。受戊型肝炎病毒感染的猪肉产品可能与病例增加有关，但日本市场上猪肉中戊型肝炎病毒的相关信息有限。2020年2月至3月，从14个农场运来的200头屠宰猪中采集胆囊，采用逆转录定量聚合酶链反应检测胆汁中的戊型肝炎病毒RNA。然后对样本进行测序和基因分型。20头猪的戊型肝炎核糖核酸呈阳性，7个样本的Ct值小于30。在这20头猪中，14头猪的病毒株被确定为3型。本报告表明，受戊型肝炎病毒污染的猪肝被运往消费市场，并证明了在待运输肉类中检测戊型肝炎病毒的重要性。
