State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China.
State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036 , China.
Tree Physiol. 2023 May 12;43(5):867-878. doi: 10.1093/treephys/tpad004.
As a critical signaling molecule, ABA plays an important role in plant growth, development and stresses response. However, tea plant [Camellia sinensis (L.)], an important economical perennial woody plant, has not been systematically reported in response to ABA signal transduction in vivo. In this study, we mined and identified the gene structure of CsPYL/CsPP2C-A/CsSnRK gene families in the ABA signal transduction pathway through the genome-wide analysis of tea plants. Spatiotemporal expression and stress response (drought, salt, chilling) expression patterns were characterized. The results showed that most members of CsPYLs were conserved, and the gene structures of members of A-type CsPP2Cs were highly similar, whereas the gene structure of CsSnRK2s was highly variable. The transcription levels of different family members were differentially expressed with plant growth and development, and their response to stress signal patterns was highly correlated. The expression patterns of CsPYL/CsPP2C-A/CsSnRK2 gene family members in different tissues of tea plant cuttings after exogenous ABA treatment were detected by qRT-PCR, and the hierarchical model of ABA signaling was constructed by correlation analysis to preliminarily obtain three potential ABA-dependent signaling transduction pathways. Subsequently, the protein interaction of the CsPYL4/7-CsPP2C-A2-CsSnRK2.8 signaling pathway was verified by yeast two-hybrid and surface plasmon resonance experiments, indicating that there is specific selectivity in the ABA signaling pathway. Our results provided novel insights into the ABA-dependent signal transduction model in tea plant and information for future functional characterizations of stress tolerance genes in tea plant.
作为一种关键的信号分子，ABA 在植物生长、发育和应激反应中发挥着重要作用。然而，茶树（Camellia sinensis (L.)）作为一种重要的经济多年生木本植物，其 ABA 信号转导的体内反应尚未得到系统报道。在本研究中，我们通过茶树基因组的全基因组分析，挖掘并鉴定了 ABA 信号转导途径中 CsPYL/CsPP2C-A/CsSnRK 基因家族的基因结构。对其时空表达和胁迫（干旱、盐、冷）响应表达模式进行了分析。结果表明，大多数 CsPYL 成员是保守的，A 型 CsPP2C 成员的基因结构高度相似，而 CsSnRK2 基因结构高度可变。不同家族成员的转录水平随植物生长发育而差异表达，其对胁迫信号模式的响应高度相关。通过 qRT-PCR 检测了外源 ABA 处理后茶树插条不同组织中 CsPYL/CsPP2C-A/CsSnRK2 基因家族成员的表达模式，通过相关性分析构建了 ABA 信号的层次模型，初步获得了三条潜在的 ABA 依赖的信号转导途径。随后，通过酵母双杂交和表面等离子体共振实验验证了 CsPYL4/7-CsPP2C-A2-CsSnRK2.8 信号通路的蛋白互作，表明 ABA 信号通路存在特异性选择。我们的研究结果为茶树 ABA 依赖的信号转导模型提供了新的见解，并为茶树未来的抗逆基因功能特性研究提供了信息。
