State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070, China.
College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, China.
BMC Genomics. 2022 Aug 6;23(1):563. doi: 10.1186/s12864-022-08734-y.
Type 2C protein phosphatase (PP2C) is a negative regulator of ABA signaling pathway, which plays important roles in stress signal transduction in plants. However, little research on the PP2C genes family of cucumber (Cucumis sativus L.), as an important economic vegetable, has been conducted.
This study conducted a genome-wide investigation of the CsPP2C gene family. Through bioinformatics analysis, 56 CsPP2C genes were identified in cucumber. Based on phylogenetic analysis, the PP2C genes of cucumber and Arabidopsis were divided into 13 groups. Gene structure and conserved motif analysis showed that CsPP2C genes in the same group had similar gene structure and conserved domains. Collinearity analysis showed that segmental duplication events played a key role in the expansion of the cucumber PP2C genes family. In addition, the expression of CsPP2Cs under different abiotic treatments was analyzed by qRT-PCR. The results reveal that CsPP2C family genes showed different expression patterns under ABA, drought, salt, and cold treatment, and that CsPP2C3, 11-17, 23, 45, 54 and 55 responded significantly to the four stresses. By predicting the cis-elements in the promoter, we found that all CsPP2C members contained ABA response elements and drought response elements. Additionally, the expression patterns of CsPP2C genes were specific in different tissues.
The results of this study provide a reference for the genome-wide identification of the PP2C gene family in other species and provide a basis for future studies on the function of PP2C genes in cucumber.
2C 型蛋白磷酸酶（PP2C）是 ABA 信号通路的负调控因子，在植物应激信号转导中发挥重要作用。然而，作为一种重要的经济蔬菜，黄瓜（Cucumis sativus L.）PP2C 基因家族的研究甚少。
本研究对黄瓜 CsPP2C 基因家族进行了全基因组研究。通过生物信息学分析，在黄瓜中鉴定出 56 个 CsPP2C 基因。基于系统进化分析，将黄瓜和拟南芥的 PP2C 基因分为 13 组。基因结构和保守基序分析表明，黄瓜 PP2C 基因在同一组中具有相似的基因结构和保守结构域。共线性分析表明，片段复制事件在黄瓜 PP2C 基因家族的扩张中发挥了关键作用。此外，通过 qRT-PCR 分析了 CsPP2C 基因在不同非生物处理下的表达。结果表明，CsPP2C 家族基因在 ABA、干旱、盐和冷胁迫下表现出不同的表达模式，CsPP2C3、11-17、23、45、54 和 55 对四种胁迫的响应显著。通过预测启动子中的顺式作用元件，我们发现所有 CsPP2C 成员都包含 ABA 响应元件和干旱响应元件。此外，CsPP2C 基因的表达模式在不同组织中具有特异性。
本研究结果为其他物种 PP2C 基因家族的全基因组鉴定提供了参考，并为进一步研究黄瓜 PP2C 基因的功能提供了依据。
