Laboratory of Nervous System Diseases and Therapy, GIGA Neuroscience, GIGA Institute, University of Liège, Liège, Belgium.
Neurosurgery Department, University Hospital, University of Liège, Liège, Belgium.
J Neurooncol. 2023 Feb;161(3):515-523. doi: 10.1007/s11060-023-04240-7. Epub 2023 Jan 25.
In this work, we aimed to comprehensively document the expression of Strawberry Notch homolog (SBNO) 1 and 2 in glioblastoma (GBM) tissue and patient-derived GBM stem-like cell (GSC) cultures.
We investigated SBNO1 and SBNO2 expression at the RNA and protein levels in glioma patient tissue and GSCs, respectively by performing immunostainings and qPCR analyses. We also used publicly-available datasets for assessing SBNO1 and SBNO2 gene expression and related copy number alterations. We used lentiviral transduction of SBNO2 to analyze the effect of its expression in patient-derived GSCs.
We observed that SBNO2 expression is increased in GBM tissue samples compared to non tumoral brain, or lower-grade gliomas, whereas SBNO1 expression remains unchanged. We hypothesized that such SBNO2 high expression might be linked to copy-number alterations at the level of the 19p13 chromosome section. We located SBNO1 and SBNO2 in different subcellular compartments. Finally, we observed that SBNO2 overexpression induces different phenotypes in different patient-derived GSCs.
These results provide the first characterization of SBNO1 and SBNO2 expression in glioma tissue, and indicate SBNO2 as highly expressed in GBM.
在这项工作中，我们旨在全面记录草莓 Notch 同源物（SBNO）1 和 2 在胶质母细胞瘤（GBM）组织和患者来源的 GBM 干细胞样细胞（GSC）培养物中的表达。
我们通过免疫染色和 qPCR 分析分别研究了 SBNO1 和 SBNO2 在胶质瘤患者组织和 GSCs 中的 RNA 和蛋白质水平的表达。我们还使用了公开可用的数据集来评估 SBNO1 和 SBNO2 基因表达和相关拷贝数改变。我们使用慢病毒转导 SBNO2 来分析其在患者来源的 GSCs 中的表达对其的影响。
我们观察到 SBNO2 在 GBM 组织样本中的表达高于非肿瘤性大脑或低级别胶质瘤，而 SBNO1 的表达保持不变。我们假设这种 SBNO2 的高表达可能与 19p13 染色体节段的拷贝数改变有关。我们将 SBNO1 和 SBNO2 定位在不同的亚细胞隔室中。最后，我们观察到 SBNO2 的过表达在不同的患者来源的 GSCs 中诱导不同的表型。
这些结果首次描述了 SBNO1 和 SBNO2 在胶质瘤组织中的表达，并表明 SBNO2 在 GBM 中高度表达。
