Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Department of Physiology, National Organization for Drug Control and Research, 12553, Giza, Egypt.
Metab Brain Dis. 2023 Apr;38(4):1365-1377. doi: 10.1007/s11011-023-01162-1. Epub 2023 Jan 25.
Malnutrition and low dietary protein intake could be risk factors for developing peripheral and central hyperammonemia, especially in pediatrics. Both curcumin and resveratrol proved to be effective against several hepatic and cerebral injuries. They were reported to be beneficial in lowering circulating ammonia levels, yet both are known for their low bioavailability. The use of pharmaceutical nano-formulations as delivery systems for these two nutraceuticals could solve the aforementioned problem. Hence, the present study aimed to investigate the valuable outcome of using a combination of curcumin and resveratrol in a nanoemulsion formulation, to counteract protein-deficient diet (PDD)-induced hyperammonemia and the consequent complications in male albino rats. Results revealed that using a nanoemulsion containing both curcumin and resveratrol at a dose of (5 + 5 mg/kg) effectively reduced hepatic and brain ammonia levels, serum ALT and AST levels, hepatic and brain nitric oxide levels, oxidative DNA damage as well as disrupted cellular energy performance. In addition, there was a substantial increase in brain levels of monoamines, and a decrease in glutamate content. Therefore, it can be concluded that the use of combined curcumin and resveratrol nanoemulsion is an effective means of ameliorating the hepatic and cerebral adverse effects resulting from PDD-induced hyperammonemia in rats.
营养不良和低蛋白质饮食可能是导致周围性和中枢性高血氨症的危险因素，特别是在儿科。姜黄素和白藜芦醇已被证明对多种肝和脑损伤有效。它们被报道能降低循环氨水平，但都因其生物利用度低而受到限制。使用药物纳米制剂作为这两种营养保健品的递送系统可以解决上述问题。因此，本研究旨在探讨姜黄素和白藜芦醇联合使用在纳米乳剂配方中对抗蛋白缺乏饮食（PDD）诱导的高血氨症及其在雄性白化大鼠中引起的并发症的有益结果。结果表明，使用含有姜黄素和白藜芦醇的纳米乳剂（剂量为（5 + 5 mg/kg））能有效降低肝和脑氨水平、血清 ALT 和 AST 水平、肝和脑一氧化氮水平、氧化 DNA 损伤以及破坏细胞能量性能。此外，脑中单胺类物质水平显著升高，谷氨酸含量降低。因此，可以得出结论，使用联合姜黄素和白藜芦醇纳米乳剂是一种有效的方法，可以改善 PDD 诱导的高血氨症引起的大鼠肝和脑的不良反应。
