Tang Hehe, Liu Dapeng, Zhang Huiling, Fan Wenlei, Hu Jian, Xu Yaxi, Guo Zhanbao, Huang Wei, Hou Shuisheng, Zhou Zhengkui
Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Shandong Rongda Agricultural Development Co. Ltd, Liaocheng, China.
Anim Genet. 2023 Jun;54(3):363-374. doi: 10.1111/age.13297. Epub 2023 Jan 25.
The thickness of the perimysium has an essential effect on the tenderness of the meat. However, the genetic basis underlying perimysial thickness has not been determined. The objective of this study was to explore the quantitative trait loci (QTL) that influence perimysial thickness in an F segregating population generated by Mallard × Pekin duck using the genome-wide association study (GWAS) method. Two QTL identified in chromosomes 27 and 13 displayed significant associations with perimysial thickness traits at the genome-wide level. The strongest association was the QTL located in chromosome 27, and this region had an effect on perimysial thickness and contained a promising candidate gene MAGI3 (Membrane-associated guanylate kinase, WW and PDZ domain containing 3). Meanwhile, association analysis showed that the top SNP within the MAGI3 gene was also associated with intramuscular fat content traits, which showed that perimysial thickness was positively correlated with intramuscular fat content. The second strongest association was the QTL region of chromosome 13. SUCLG2 (Succinate-CoA ligase GDP-forming subunit beta) is proximal to the top SNP and stood out as another candidate gene. Furthermore, the Transposase-Accessible Chromatin using Sequencing result showed that some key transcription factors (MYF5, MYOD1, KLF11) related to muscle development or energy metabolism might bind to the open regions of MAGI3 and SUCLG2. By analyzing the expression of different genotypes of the candidate gene, we speculate that different genotypes of MAGI3 may have an effect on breast muscle development, and then affect the thickness of the perimysium. This study maps two major genes of the duck breast muscle perimysial thickness trait, which helps to characterize muscle development and contributes to the genetic improvement of meat yield and quality in livestock.
肌束膜的厚度对肉的嫩度有重要影响。然而，肌束膜厚度的遗传基础尚未确定。本研究的目的是利用全基因组关联研究（GWAS）方法，在由绿头鸭×北京鸭产生的F分离群体中探索影响肌束膜厚度的数量性状位点（QTL）。在27号和13号染色体上鉴定出的两个QTL在全基因组水平上与肌束膜厚度性状表现出显著关联。最强的关联是位于27号染色体上的QTL，该区域对肌束膜厚度有影响，并且包含一个有前景的候选基因MAGI3（含膜相关鸟苷酸激酶、WW和PDZ结构域3）。同时，关联分析表明MAGI3基因内的顶级单核苷酸多态性（SNP）也与肌内脂肪含量性状相关，这表明肌束膜厚度与肌内脂肪含量呈正相关。第二强的关联是13号染色体的QTL区域。琥珀酰辅酶A连接酶GDP形成亚基β（SUCLG2）靠近顶级SNP，并作为另一个候选基因脱颖而出。此外，利用测序技术进行的转座酶可及染色质结果显示，一些与肌肉发育或能量代谢相关的关键转录因子（MYF5、MYOD1、KLF11）可能与MAGI3和SUCLG2的开放区域结合。通过分析候选基因不同基因型的表达，我们推测MAGI3的不同基因型可能对胸肌发育有影响，进而影响肌束膜的厚度。本研究定位了鸭胸肌肌束膜厚度性状的两个主要基因，这有助于表征肌肉发育，并有助于家畜肉产量和品质的遗传改良。
