全基因组关联研究 Pekin 鸭血液成分水平。

Genome-wide association study of the level of blood components in Pekin ducks.

机构信息

National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, MARA, Department of Animal Genetics and Breeding, China Agricultural University, Beijing 100193, China.

Beijing General Station of Animal Husbandry, Beijing 100107, China.

出版信息

Genomics. 2020 Jan;112(1):379-387. doi: 10.1016/j.ygeno.2019.02.017. Epub 2019 Feb 25.

DOI:10.1016/j.ygeno.2019.02.017

PMID:30818062

Abstract

Blood components are considered to reflect nutrient metabolism and immune activity in both humans and animals. In this study, we measured 12 blood components in Pekin ducks and performed genome-wide association analysis to identify the QTLs (quantitative trait locus) using a genotyping-by-sequencing strategy. A total of 54 QTLs were identified for blood components. One genome-wide significant QTL for alkaline phosphatase was identified within the intron-region of the OTOG gene (P = 1.31E-07). Moreover, 21 genome-wide significant SNPs for the level of serum cholinesterase were identified on six different scaffolds. In addition, for serum calcium, one genome-wide significant QTL was identified in the upstream region of gene RAB11B. These results provide new markers for functional studies in Pekin ducks, and several candidate genes were identified, which may provide additional insights into specific mechanisms for blood metabolism in ducks and their potential application for duck breeding programs.

摘要

血液成分被认为反映了人类和动物的营养代谢和免疫活性。在这项研究中，我们测量了北京鸭的 12 种血液成分，并使用测序基因分型策略进行全基因组关联分析，以鉴定数量性状基因座（QTL）。总共鉴定出 54 个与血液成分相关的 QTL。碱性磷酸酶的一个全基因组显著 QTL 位于 OTOG 基因的内含子区域（P=1.31E-07）。此外，在六个不同的支架上鉴定出了 21 个与血清胆碱酯酶水平相关的全基因组显著 SNP。此外，对于血清钙，在 RAB11B 基因的上游区域鉴定出了一个全基因组显著的 QTL。这些结果为北京鸭的功能研究提供了新的标记，鉴定出了几个候选基因，这可能为鸭子血液代谢的特定机制及其在鸭养殖计划中的潜在应用提供了更多的见解。