Evinger-Hodges M J, Dicke K A, Gutterman J U, Blick M
Department of Hematology, U.T. M.D. Anderson Hospital & Tumor Institute, Houston 77030.
Leukemia. 1987 Aug;1(8):597-602.
We and other investigators have previously reported our findings on oncogene expression in human leukemia in an attempt to study the possible involvement of these genes in the leukemic state. An important shortcoming of these studies has been the lack of information on the expression of these genes in normal hematopoietic cells. To address this question we analyzed both the transcript size and level of expression of six oncogenes in fresh hematopoietic cells obtained from hematologically normal individuals and compared the results to those found in fresh samples obtained from patients with various forms of leukemia (acute myelogenous leukemia, acute lymphocytic leukemia, and chronic myelogenous leukemia). We found low level expression of c-myc, c-myb, c-fes, and c-raf in normal bone marrow in sharp contrast to the high levels of expression found in some forms of leukemia. C-fos was highly expressed in both normal bone marrow and certain leukemias. We were unable to detect c-sis expression in our normal samples. With the exception of c-fes, there was no variation in transcript size when comparing normal and leukemic samples. Having defined the transcript sizes and levels of expression for these proto-oncogenes in normal hematopoietic cells, we know that aberrant transcript size for the genes we have studied is not a common event in leukemias. The levels of expression, however, vary widely between normal hematopoietic cells and leukemia as well as between different types of leukemia.
我们和其他研究人员此前曾报告过我们在人类白血病中癌基因表达方面的研究结果，试图探究这些基因在白血病状态中可能发挥的作用。这些研究的一个重要缺陷是缺乏关于这些基因在正常造血细胞中表达情况的信息。为了解决这个问题，我们分析了从血液学正常个体获取的新鲜造血细胞中六个癌基因的转录本大小和表达水平，并将结果与从各种白血病（急性髓性白血病、急性淋巴细胞白血病和慢性髓性白血病）患者获取的新鲜样本中的结果进行比较。我们发现，与某些形式白血病中发现的高表达水平形成鲜明对比的是，正常骨髓中c-myc、c-myb、c-fes和c-raf呈低水平表达。C-fos在正常骨髓和某些白血病中均高表达。在我们的正常样本中未检测到c-sis的表达。除c-fes外，正常样本与白血病样本比较时转录本大小没有差异。在确定了这些原癌基因在正常造血细胞中的转录本大小和表达水平后，我们知道在所研究基因中，转录本大小异常在白血病中并非常见情况。然而，正常造血细胞与白血病之间以及不同类型白血病之间的表达水平差异很大。
