Lee J, Talpaz M, Walters R, Gutterman J U, Blick M
Department of Hematology, University of Texas M.D. Anderson Hospital and Tumor Institute, Houston.
Hematol Pathol. 1988;2(4):229-37.
The proto-oncogenes have the capacity for conversion to an oncogene that is capable of inducing or maintaining the transformed state when they are overly expressed or altered by mutation or rearrangement. To study the possible involvement of these genes in the neoplastic transformation of B-cell malignancies, we have analyzed their expression in 18 fresh samples obtained from the peripheral blood of patients with a variety of B-cell malignancies. A high-molecular weight c-k-ras transcript (5.2 kb) was detected in all samples including normal lymphocytes. In contrast, a low-molecular weight c-k-ras transcript (1.2 kb) was not detected in our control normal lymphocytes, but was found in 9 fresh samples. The 1.2 kb c-k-ras transcript was expressed 2-3-fold higher than the 5.2 kb c-k-ras transcript. C-h-ras (2.9 kb, 1.4 kb) was expressed at a low level in 4 and 13 samples, but not in normal lymphocytes. C-myc (2.3 kb) and c-raf (3.8 kb) were expressed in all samples including normal lymphocytes without significant variation in the level of expression. C-fos (2.2 kb) was expressed in 15 samples and normal lymphocytes with a wide range in the level of expression. C-myb (3.7 kb) and c-fes (2.7 kb) were expressed at a low level in 6 and 10 samples, respectively, including normal lymphocytes. C-sis (4.0 kb) was not detected in any sample including normal lymphocytes.
原癌基因在过度表达、因突变或重排而改变时，具有转变为癌基因的能力，该癌基因能够诱导或维持细胞的转化状态。为了研究这些基因在B细胞恶性肿瘤的肿瘤转化中可能的作用，我们分析了从患有各种B细胞恶性肿瘤的患者外周血中获取的18份新鲜样本中它们的表达情况。在包括正常淋巴细胞在内的所有样本中均检测到高分子量的c-k-ras转录本（5.2 kb）。相比之下，低分子量的c-k-ras转录本（1.2 kb）在我们的对照正常淋巴细胞中未检测到，但在9份新鲜样本中被发现。1.2 kb的c-k-ras转录本的表达量比5.2 kb的c-k-ras转录本高2至3倍。C-h-ras（2.9 kb、1.4 kb）在4份和13份样本中低水平表达，但在正常淋巴细胞中不表达。C-myc（2.3 kb）和c-raf（3.8 kb）在包括正常淋巴细胞在内的所有样本中均有表达，表达水平无明显差异。C-fos（2.2 kb）在15份样本和正常淋巴细胞中有表达，表达水平范围较广。C-myb（3.7 kb）和c-fes（2.7 kb）分别在6份和10份样本中低水平表达，包括正常淋巴细胞。在包括正常淋巴细胞在内的任何样本中均未检测到C-sis（4.0 kb）。
