Ndosi Barakaeli Abdieli, Lee Dongmin, Bia Mohammed Mebarek, Yang Heejae, Hong Min-Ji, Seo Sungbo, Park Hansol, Eom Keeseon S
Department of Parasitology, Parasitology Research Center and International Parasite Resource Bank, Chungbuk National University, School of Medicine, Cheongju 28644, Republic of Korea.
Tanzania Wildlife Management Authority, P.O. Box 2658 Morogoro, Tanzania.
J Parasitol Res. 2023 Jan 16;2023:1923804. doi: 10.1155/2023/1923804. eCollection 2023.
The genus is the major abomasal parasite of ruminants responsible for substantial economic losses in tropical and temperate regions. This study was conducted to clarify the morphometry and molecular characterisation of species isolated from sheep in Babati district, Tanzania. A total of 486 trichostrongylid nematodes were recovered from five sheep. Of the total worms, 106 nematodes were distinguished by 37 males and 69 females. The asymmetrical length of dorsal ray and the distance of bulb at the apex of spicules were used for identification of males. In females, the linguiform vulvar flap was the most predominant with 33 out of 69 (48%) compared with knobbed morph type which was 25/69 (36%) and smooth morph type with 11/69 (16%). Partial 1 sequence fragments of isolates showed 98.8%, 99.3%, 99.7%, 99.5%, 99.3%, and 98.4% in male, smooth, knobbed, linguiform A, linguiform B, and linguiform C, respectively; with the average nucleotide divergence ranged from 1.03 to 2.35%. The amplified fragments of ITS-2 genes in knobbed, linguiform A, and smooth morphotypes revealed 99.4%, 98.5%, and 98.3%, respectively. Phylogenetic analysis was evaluated by employing Bayesian inference and maximum-likelihood, and the tree was distinctly separated into three clusters focusing on in cluster I within the family Haemonchidae. Genetic drifting, mutation, and modification of the morphological features of the species described to have an impact on the development of drug resistance. Species identification is necessary to understand which species infect animal host. We recommend more studies on the parasites intensity and the strategies for controlling species in Tanzania.
该属是反刍动物皱胃的主要寄生虫，在热带和温带地区造成重大经济损失。本研究旨在阐明从坦桑尼亚巴巴蒂区绵羊分离出的该属物种的形态测量和分子特征。从五只绵羊中总共回收了486条毛圆线虫。在所有蠕虫中，106条线虫被鉴定出来，其中雄性37条，雌性69条。背射线的不对称长度和交合刺顶端球状体的距离用于雄性鉴定。在雌性中，舌状阴门瓣最为常见，69条中有33条（48%），相比之下，有瘤形态类型为25/69（36%），光滑形态类型为11/69（16%）。该属分离株的部分1序列片段在雄性、光滑型、有瘤型、舌状A、舌状B和舌状C中分别显示为98.8%、99.3%、99.7%、99.5%、99.3%和98.4%；平均核苷酸差异范围为1.03%至2.35%。有瘤型、舌状A和光滑形态型的ITS-2基因扩增片段分别显示为99.4%、98.5%和98.3%。采用贝叶斯推断和最大似然法进行系统发育分析，该树明显分为三个簇，重点是血矛线虫科内簇I中的该属。所述该属物种的遗传漂移、突变和形态特征改变对耐药性发展有影响。物种鉴定对于了解哪些物种感染动物宿主很有必要。我们建议对坦桑尼亚的寄生虫感染强度和控制该属物种的策略进行更多研究。
