Hussain Akbar Jaber, Behbehani Jawad, Karched Maribasappa
Department of Restorative Sciences, Faculty of Dentistry, Kuwait University, Kuwait City, Kuwait.
Department of Bioclinical Sciences, Faculty of Dentistry, Kuwait University, Kuwait City, Kuwait.
Front Oral Health. 2023 Jan 9;3:1071018. doi: 10.3389/froh.2022.1071018. eCollection 2022.
Biofilm formation in dental unit waterlines and the resulting microbial contamination of the water in the system has become a significant problem. Contaminated water in the dental units is a major concern in dental clinics due to potential risk of causing infections particularly in elderly and immunocompromised patients. The aim of this study was at first to determine microbial contamination of the dental unit waterlines and then to study the efficacy of a comprehensive disinfection protocol on decreasing the microbial load. Water samples were collected before and after disinfection procedure from handpieces and water storage bottles from the dental units, a small 1-cm tubing was cut from each unit and subjected to microbiological culture on different growth media. Identification of the predominant species was achieved by 16S rRNA gene sequencing. Microbial growth was observed in samples collected from all dental units. Upon disinfection procedure, microbial contamination in the water samples and in the tubing surfaces was significantly reduced ( > 0.05). 16S rRNA gene sequencing revealed the presence of several species belonging to the genera , and , some of which are implicated in human infections. Aggravation of the biofilm growth on the tubing surfaces and the microbial contamination in the water can be effectively controlled by implementing appropriate and routine disinfection protocols. This may help protect the dental unit staff and the patients being exposed to the risk of infections.
牙科设备水线中的生物膜形成以及由此导致的系统内水的微生物污染已成为一个重大问题。牙科设备中的污染水是牙科诊所的主要关注点，因为存在引发感染的潜在风险，尤其是在老年患者和免疫功能低下的患者中。本研究的目的首先是确定牙科设备水线的微生物污染情况，然后研究一种综合消毒方案在降低微生物负荷方面的效果。在消毒程序前后，从牙科设备的机头和储水瓶中采集水样，从每个设备中切下一小段1厘米长的管道，并在不同的生长培养基上进行微生物培养。通过16S rRNA基因测序鉴定主要菌种。在从所有牙科设备采集的样本中均观察到微生物生长。经过消毒程序后，水样和管道表面的微生物污染显著减少（>0.05）。16S rRNA基因测序显示存在属于 、 和 属的几种菌种，其中一些与人类感染有关。通过实施适当的常规消毒方案，可以有效控制管道表面生物膜生长的加剧以及水中的微生物污染。这可能有助于保护牙科设备工作人员和面临感染风险的患者。
